17
25 x KPSS (1000ml)
Solution 1:
Chemical
MW (g/mol)
Conc. (mmol/l) Conc. (g/l)
KCl
74.56
123.70
230.575
CaCl
2
-
2
H
2
O
147.02
2.50
9.200
Solution 2:
Chemical
MW (g/mol)
Conc. (mmol/l) Conc. (g/l)
MgSO
4
-
7
H
2
O
246.48
1.17
7.225
KH
2
PO
4
136.09
1.18
4.025
Solution 3:
Chemical
MW (g/mol)
Conc. (mmol/l) Conc. (g/l)
EDTA
372.24
0.03
0.250
1. Dissolve the chemicals for solution 1 in about 800ml double distilled H
2
O in a 1000 ml graduated.
Dissolve the chemicals for solutions 2 and
2. 3 in 75 ml double distilled H
2
O in individually cylinders. Gently heat solution 3 to dissolve the EDTA.
3. Solutions 2 and 3 are added to solution 1 and the graduated bottle is filled with additional double
distilled H
2
O to reach a final volume of 1000 ml.
Before use:
4. Dilute the 25 x PSS stock solution 1:25 using double distilled H
2
O.
5. Add
1.091 g/l Glucose
2.100 g/l NaHCO
3
6. Aerate the solution with carbogen (95% O
2
+ 5% CO
2
) for at least 20 minutes. If necessary wait for the
pH of the buffer to reach pH 7.4.
Summary of Contents for 750 TOBS
Page 1: ...USER GUIDE VOL 2 0 TISSUE ORGAN BATH SYSTEM 750TOBS ...
Page 2: ......
