Dionex AS-HV Operator'S Manual Download Page 13

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Doc. 065125-01 10/06

2 • Description

2.1

AS-HV Overview

Figure 2-1

is an overview of the main features of the AS-HV Autosampler.

Figure 2-1. AS-HV Autosampler

Power

LED

Rinse Pump

Fixed Rinse

Reservoir

Sample Rack

Rack Location Mat

Base Support Tube

Rinse Pump

Speed Knob

Standards Rack

Sampling Arm
Support

Sampling Needle

Guide

Sampling Arm

Nunclon Sample
Flasks

Summary of Contents for AS-HV

Page 1: ...AS HV Autosampler Operator s Manual Document No 065125 Revision 01 October 2006...

Page 2: ...S OF THE USE OF THIS PUBLICATION IN TERMS OF CORRECTNESS ACCURACY RELIABILITY CURRENTNESS OR OTHERWISE FURTHER DIONEX CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION AND TO MAKE CHANGES FROM...

Page 3: ...Notes 3 1 3 Safety Labels 4 2 Description 2 1 AS HV Overview 5 2 2 AS HV Side Panel 8 2 3 Sample Loading Modes 12 2 4 Sample Loading Pumps 13 2 4 1 Syringe Pump 13 2 4 2 AXP Pump 14 2 4 3 External Pe...

Page 4: ...Program 25 3 3 Routine Maintenance 37 3 3 1 Daily Maintenance 37 3 3 2 Weekly Maintenance 38 3 3 3 Monthly Maintenance 38 3 3 4 Periodic Maintenance 38 4 Troubleshooting 4 1 Liquid Leaks 39 4 1 1 Lea...

Page 5: ...w Syringe 50 5 2 4 Initializing the New Syringe 51 5 3 Replacing the Sampling Needle 52 5 3 1 Removing the Existing Sampling Needle 52 5 3 2 Installing the New Sampling Needle 53 5 4 Replacing the Syr...

Page 6: ...ng the Sample Rack 67 B 3 7 Installing the Trace Analysis Cover Optional 67 B 3 8 Installing the Sampling Needle 68 B 3 9 Connecting the Syringe Pump Optional 71 B 3 10 Connecting the AXP Pump Optiona...

Page 7: ...Contents Doc 065125 01 10 06 v C Error Messages and Codes C 1 Audit Trail Messages 85 C 2 AS HV ERROR Indicator Codes 107 D Reordering Information...

Page 8: ...AS HV Operator s Manual vi Doc 065125 01 10 06...

Page 9: ...position standards rack for loading of standards and or samples A PEEK sampling needle for aspirating sample from sample containers A sampling needle guide that prevents the PEEK sampling needle from...

Page 10: ...inning operation instructions for creating Chromeleon programs for AS HV control and routine preventive maintenance requirements Chapter 4 Troubleshooting Minor problems that may occur during operatio...

Page 11: ...hazardous situation which if not avoided may result in minor or moderate injury Indicates that the function or process of the instrument may be impaired Operation does not constitute a hazard Signale...

Page 12: ...C22 2 No 61010 1 2004 safety UL 61010 1 2004 safety EN 61326 1997 A1 1998 A2 2001 EMC Low Voltage Equipment Directive 73 23 EEC and EMC Directive 89 336 EEC These symbols appear on the AS HV or on AS...

Page 13: ...ain features of the AS HV Autosampler Figure 2 1 AS HV Autosampler Power LED Rinse Pump Fixed Rinse Reservoir Sample Rack Rack Location Mat Base Support Tube Rinse Pump Speed Knob Standards Rack Sampl...

Page 14: ...n a split septum that allows the PEEK sampling needle to pierce the cap while also maintaining sample integrity and minimizing sample evaporation Optional A 15 position sample rack P N 064234 that acc...

Page 15: ...To ensure that the PEEK sampling needle does not bend or flex as it pierces the septum in the sample container cap To ensure that the sample container is not lifted out of the sample rack during sampl...

Page 16: ...vement in the X Theta and Z axes see Figure 3 1 Successful completion of the reset procedure confirms that the motors operate and the action of the carriages is correct A small pointed device is requi...

Page 17: ...relay is energized the corresponding indicator is lit The relay outputs can be used to connect and control low power devices These outputs should be limited to loads of 1 amp or less The eight digita...

Page 18: ...is not available on the PC order the optional RS 232 to USB cable P N 064261 and use this cable to connect the autosampler to a USB port on the PC Power Entry The power entry incorporates the AS HV ma...

Page 19: ...ement accessible STROMSCHLAGGEFAHR Zur Vermeidung von elektrischen Schl gen ist eine geerdete Steckdose zu verwenden Das Ger t darf nicht ohne Erdung betrieben bzw an Wechselstrom angeschlossen werden...

Page 20: ...HV support overlapping sample preparation This means that while data collection and analysis for the current sample are still in progress the AS HV can perform sample preparation steps for the next sa...

Page 21: ...ump the AS HV is capable of both concentrator and loop loading in the push or pull mode see Section 2 3 This configuration provides maximum flexibility in terms of Chromeleon control and flow precisio...

Page 22: ...us flow of liquid through the pump With the AXP pump these problems are most likely to occur when performing concentrator loading in the pull mode To ensure optimal performance when doing concentrator...

Page 23: ...doing loop loading with the external peristaltic pump follow these guidelines All points in the system that will be aspirated should be plumbed with 1 0 mm 0 040 in ID PEEK tubing P N 054410 To ensure...

Page 24: ...ormance when using the internal peristaltic pump for sample loading the delivery pressure should be less than 0 03 MPa 5 psi 2 4 5 Relay Controlled Pump An external relay controlled pump from a third...

Page 25: ...g pumps see Table 2 1 through Table 2 4 You can select these commands from the default Control panel or the Commands dialog box To open the dialog box press F8 or select Control Command Command Functi...

Page 26: ...ntSetup Reports the AXP pump configuration and the status of several operating parameters read only To view details click the plus sign beside this command AxpState Turns the AXP pump on and off Flow...

Page 27: ...se fluid used to wash the sample loop sampling needle and sample loading path This command is not available unless the internal peristaltic pump is selected as the Sample Loading Pump in the AS HV Pro...

Page 28: ...AS HV Operator s Manual 20 Doc 065125 01 10 06...

Page 29: ...mains without an earthed ground connection The power supply cord is used as the main disconnect device Make sure the socket outlet is located near the AS HV and is easily accessible Operation at AC i...

Page 30: ...P N 064235 pkg of 50 Nalgene bottle caps and septa P N 064232 pkg of 72 as follows a Turn one of the sample container caps upside down Place a septum white side facing down in the cap Push the septum...

Page 31: ...p the AS HV automatically begins an initialization sequence in which the sampling needle moves through the extent of the autosampler s working envelope Upon completing the initialization sequence the...

Page 32: ...on see Figure 3 2 The default speed is nominally set to obtain a minimum speed of 40 rpm and a maximum of 100 rpm When pumping viscous fluids run at a slow speed Figure 3 2 Rinse Pump Speed Control Kn...

Page 33: ...or user s guide To create a program 1 Start the Chromeleon Server if it is not already running If the Chromeleon Server icon on the Windows taskbar is crossed out in red the Server is not running Sta...

Page 34: ...V screens On each screen select the required parameters and click Next until the Sampler Options screen is displayed 7 There are three versions of the Sampler Options screen The version that appears n...

Page 35: ...p group box There are two syringe speeds fast and slow and each speed has 10 levels The actual speed depends on multiple factors for details refer to the following sections Fast Syringe Speed The syri...

Page 36: ...ecting a speed setting be sure to take into account the tubing bore Table 3 1 Fast Syringe Speed Settings Speed Setting Top Speed steps sec Flow Rate mL min 1 800 10 0 2 1200 15 0 3 1600 20 0 4 2000 2...

Page 37: ...n 1 80 1 00 2 160 2 00 3 200 2 50 4 240 3 00 5 280 3 50 6 320 4 00 7 360 4 50 8 400 5 00 9 440 5 50 10 480 6 00 Table 3 4 Slow Syringe Speed Settings Sample Aspirate Speed for Sample Loop Push or Pull...

Page 38: ...eps sec Flow Rate mL min 1 40 0 50 2 80 1 00 3 100 1 25 4 120 1 50 5 140 1 75 6 160 2 00 7 180 2 25 8 200 2 50 9 220 2 75 10 240 3 00 Table 3 6 Slow Syringe Speed Settings Sample Aspirate Speed for Co...

Page 39: ...on occurs This setting is ignored unless you select Sample Loop as the sample loading type in the Server Configuration see Section B 3 16 NOTE For sample overlap programs the Injection Duration must b...

Page 40: ...he AXP Pump group box Select the Rinse Volume Select the Flow Rate Set the Low Pressure Limit The maximum value is the current upper pressure limit setting in psi minus 100 the minimum value is 0 Set...

Page 41: ...e loading pump is either the internal peristaltic pump or a relay controlled pump this screen is displayed Select the Needle Height Select the Rinse Volume In the Internal Peristaltic Pump Relay Contr...

Page 42: ...the device page for a particular module the list box on the Relay and State Devices Options page already contains this information If you change any settings here Chromeleon will automatically copy t...

Page 43: ...r the duration of the selected state in the edit box NOTE Relays A and B are reserved for internal use and cannot be selected 11 To add the following information to the list box click Add Retention Ti...

Page 44: ...icking Finish opens a dialog box in which you can save the program To review the program before saving it select Review the program in a new window Clicking Finish opens the program in the Program Edi...

Page 45: ...her components 3 3 1 Daily Maintenance Wipe up all spills immediately especially spills of corrosive liquids Inspect the syringe pump for leaks and correct any problems Flush the syringe pump thorough...

Page 46: ...n 5 1 3 3 4 Periodic Maintenance Clean the AS HV external covers spill tray rack location mat and accessories with a damp soft cloth and diluted mild detergent When operating the syringe pump in the p...

Page 47: ...to troubleshoot them Section C 2 contains a description of error codes that may be displayed by the AS HV ERROR indicators and details about how to troubleshoot them If you are unable to solve a prob...

Page 48: ...ckwise as viewed from the top see Figure 4 1 Tighten fingertight only do not overtighten Figure 4 1 Tightening the Syringe 4 1 3 Leaking Waste Line Fixed Rinse Reservoir Check that the waste line is p...

Page 49: ...y have hit an obstacle while moving into position The arm may have stopped because it failed to reach a position within the defined limits of the intended position Movement errors of less than 1 mm wi...

Page 50: ...orrect travel to determine whether there is any mechanical obstruction 3 If you do not find any mechanical obstructions turn on the AS HV power If the autosampler again fails to initialize contact Dio...

Page 51: ...HV electronics cannot be serviced by the user All repairs of the electronics components must be performed by Dionex personnel 4 5 CPU Activity LED Fails to Light To troubleshoot 1 Initialize the AS HV...

Page 52: ...ructions refer to Section 5 5 3 If the autosampler still fails to power up contact Dionex for assistance NOTE Access to the power supply unit and components requires removal of the main housing Do not...

Page 53: ...ccur when the syringe pump is operating in the pull mode To troubleshoot 1 The flow rate may be too high Reduce the flow rate 2 The concentrator column may be plugged Refer to the column manual for tr...

Page 54: ...AS HV Operator s Manual 46 Doc 065125 01 10 06...

Page 55: ...se of the problem 5 1 Cleaning the Syringe Pump To remove precipitates salts etc and eliminate bacterial growth clean the fluid path of the syringe pump monthly 1 Make a solution of 10 methanol MeOH b...

Page 56: ...Refer to Figure 5 1 when removing the existing syringe 1 Remove the liquid from the syringe 2 Remove the plunger lock screw 3 In Chromeleon select SyringeAspirate in the Commands dialog box Select the...

Page 57: ...up by slowly pulling out the plunger 4 Keep the syringe in the liquid and slowly push the syringe plunger until the bubble s exit the syringe Then fill the syringe 5 Remove the syringe from the liquid...

Page 58: ...the syringe plunger down to the plunger holder assembly 3 Screw the syringe plunger into place 4 Slide the plunger lock screw through the plunger buttons and fasten to the carriage NOTE Make sure the...

Page 59: ...the AS HV power wait 10 seconds and turn on the power again 2 In Chromeleon select SyringeReset in the Commands dialog box and click OK This moves the syringe plunger down slightly into its initial po...

Page 60: ...wrap that secures the tubing through the eyelet underneath the sampling arm housing see Figure 5 5 6 Unscrew the mounting nut on the Z axis slide and pull the sampling needle mounting block out of the...

Page 61: ...ounting block with the knurled mounting nut see Figure 5 4 3 Screw the sampling needle guide P N 064519 onto the underside of the accessories plate NOTE The sampling needle guide ensures that the PEEK...

Page 62: ...5 in from the column case see Figure 5 6 7 Attach the tubing to the middle tube anchor the anchor installed first using the two tube markers see Figure 5 6 8 Route the tubing along the side of the X...

Page 63: ...that the valve motor shaft is in the correct position a Turn off the power wait 10 seconds and turn on the power again b In the Commands dialog box select SyringeReset and click OK 3 Remove the syrin...

Page 64: ...ten from one quarter to one half turn after the screws contact the valve body 5 Pull the syringe plunger all the way into the carriage and secure by tightening the plunger lock screw NOTE When viewed...

Page 65: ...er out of the input panel see Figure 5 9 Figure 5 9 Removing the Fuse Holder 5 Remove the fuses from the fuse holder and replace with new 5 amp slow blow fuses P N 064248 Dionex recommends always repl...

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Page 67: ...ment required Typical input power 75 W Maximum line draw 1 8 A at 110 VAC at power up Fuse Two 5 amp slow blow fuses P N 064248 Operating Temperature 10 to 35 C 50 to 95 F Operating Humidity 8 to 80 r...

Page 68: ...ume Application dependent up to 250 mL Minimum Sample Volume Injection valve dependent nominally 50 L Number of Injections per Vial Variable Injection Type Full sample loop or concentrator column Inje...

Page 69: ...he AS HV and place it on the workbench 2 Unpack all items included in the shipping carton place them in a convenient location and check them against the packing list Report any discrepancies to Dionex...

Page 70: ...lignment pins protrude above the base support tube see Figure B 1 2 Position the spill tray so that the two alignment pins at the rear of the base support tube fit into the slots in the spill tray 3 L...

Page 71: ...he cutouts are designed to slot over the raised locating tabs that are molded into the spill tray this ensures proper alignment 2 Position the rack location mat over the spill tray Check that the slot...

Page 72: ...grees until it locks into position The inlet and outlet nipples on the reservoir should be facing the left hand side of the AS HV see Figure B 3 4 Verify that the rinse reservoir is seated correctly b...

Page 73: ...te the first tubing bridge into the appropriate lower tubing bridge holder Feed the tubing around the rotor and locate the center tubing bridge into the top bridge holder Make sure there are no twists...

Page 74: ...short as possible NOTE Dionex recommends using ASTM Type I or better filtered deionized water as the rinse fluid To remove tubing from the rinse pump Release the track by disengaging the track occlus...

Page 75: ...ble also Place the sample rack on top of the rack location mat in the autosampler B 3 7 Installing the Trace Analysis Cover Optional The Plexiglas trace analysis cover is shipped as a ready to assembl...

Page 76: ...4511 Sampling Needle Kit PTFE Lined and Sleeved P N 064056 passes through the accessories plate and secure it with the knurled mounting nut see Figure B 6 5 Screw the sampling needle guide P N 064519...

Page 77: ...e a loop of about 10 mm 0 39 in in the cable tie Figure B 7 Attaching Tubing to the Cable Tie Figure B 6 Installing the Sampling Needle Sampling Needle Mounting Block Z Axis Slide Mounting Nut Accesso...

Page 78: ...the middle tube anchor the anchor installed first using the two tube markers see Figure B 8 10 Route the tubing along the side of the X axis arm Anchor the tubing in position spacing the tube anchors...

Page 79: ...d lines as required for either the push mode see Figure B 9 or the pull mode see Figure B 10 4 When you finish the plumbing connections go on to Section B 3 14 Part Number Item Quantity 052112 PFA per...

Page 80: ...ample Probe Injection Valve A A 1 4 28 to 10 32 coupler double cone ferrule fitting and 10 32 double cone bolt 1 57 mm 0 062 in ID PFA Tubing 1 57 mm 0 062 in ID PFA Tubing 1 2 3 4 6 5 To Separator Co...

Page 81: ...the cable is attached to the USB port 3 To control the pump through the AS HV connect the RS 232 cable P N 060683 shipped with the AXP to the AUX port on the side panel of the autosampler NOTE To sup...

Page 82: ...jection Valve A A To Separator Column Eluent In A B The PFA tubing is in the AXP Ship Kit 1 4 28 to 10 32 coupler double cone ferrule fitting and 10 32 double cone bolt 1 2 3 4 6 5 Sample Probe Sample...

Page 83: ...mp s rear panel terminal strip 4 Connect the liquid lines as required for either the push mode see Figure B 13 or the pull mode see Figure B 14 5 When you finish the plumbing connections go on to Sect...

Page 84: ...ing on threaded side of barbed fitting The peristaltic tubing is shipped with the pump 1 0 mm 0 040 in ID PEEK Tubing To Separator Column Eluent In 1 2 3 4 6 5 Sample Probe Peristaltic Pump Peristalti...

Page 85: ...ized water source 2 Connect the liquid lines to the pump as required for the push mode see Figure B 13 or the pull mode see Figure B 14 3 When you finish the plumbing connections go on to Section B 3...

Page 86: ...l port is not available on the PC use the optional RS 232 to USB cable P N 064261 to connect the HOST port to a USB port on the computer NOTE The driver provided with the RS 232 to USB cable must be i...

Page 87: ...AC input levels outside of the specified operating voltage range may damage the AS HV DANGER D LECTROCUTION Pour viter toute lectrocution il faut utiliser une prise de courant avec prise de terre Ne...

Page 88: ...ex Ion Chromatograph IC Document No 031883 for detailed instructions In summary Turn on the computer power and log onto Windows XP or Windows 2000 as an administrator Start the Chromeleon Server if it...

Page 89: ...nstallation Doc 065125 01 10 06 81 After configuring the AS HV start the Chromeleon client Click the Default Panel Tabset toolbar button to display a set of Control panels for the devices in the timeb...

Page 90: ...ohol and slowly pull out the plunger to draw a full syringe volume into the syringe Figure B 15 Removing the Syringe 6 Keeping the syringe in the liquid slowly push the syringe plunger until the bubbl...

Page 91: ...ng the syringe at the top and bottom screw it back into the valve and tighten fingertight 11 Align the syringe piston with the connecting screw mount on the syringe drive 12 Insert the plunger lock sc...

Page 92: ...AS HV Operator s Manual 84 Doc 065125 01 10 06...

Page 93: ...blem is detected it is reported to Chromeleon and displayed in the Audit Trail Each error message is preceded by an icon that identifies the seriousness of the underlying problem see the table below Y...

Page 94: ...isconnect Abort page 90 AXP pump lower pressure limit fault Abort page 91 AXP pump motor stall fault Warning page 91 AXP pump upper pressure limit fault Abort page 91 Command timed out Abort page 92 C...

Page 95: ...100 Syringe pump plunger move not allowed Abort page 103 Syringe pump plunger overload error Abort page 103 Syringe pump valve overload error Abort page 103 Theta axis movement error Abort page 105 Th...

Page 96: ...after PrepareNextSample Cause A problem with the AS HV firmware To troubleshoot Initialize the AS HV Turn off the power wait 10 seconds and turn on the power again If the message appears again the AS...

Page 97: ...s restart the server If the message appears again contact Dionex for assistance Cause A problem with the AS HV electronics To troubleshoot Contact Dionex for assistance Cause The AS HV firmware select...

Page 98: ...ed to travel the required distance along one or more axes during the AS HV initialization sequence To troubleshoot Follow the troubleshooting steps in Section 4 2 and Section 4 3 Cause A communication...

Page 99: ...onnected to the pump s outlet becomes plugged if the pressure exceeds the maximum pressure rating of the pump or if the mechanism jams To troubleshoot Refer to the AXP pump manual for corrective actio...

Page 100: ...S HV to verify that it is in a working state Turn off the power wait 10 seconds and turn on the power again 4 Send some commands from Chromeleon to the AS HV while the commands are being sent observe...

Page 101: ...cs resulted in the sampling arm being commanded to move to a position outside of its operating envelope To troubleshoot Initialize the AS HV Turn off the power wait 10 seconds and turn on the power ag...

Page 102: ...t the baud rate is correct If the HOST LED does not flash while commands are being sent confirm that the AS HV is functioning by watching for the CPU LED to flash If the CPU LED does not start flashin...

Page 103: ...LED does not start flashing within 10 to 15 seconds press the Reset button to implement the reset sequence and then wait until the CPU LED flashes If this is successful try again to send commands fro...

Page 104: ...on sample rack and a 24 position sample rack Valid position numbers for the racks are R1 to R15 or R1 to R24 respectively Cause You entered an invalid injection volume To troubleshoot Enter a value fr...

Page 105: ...entered a parameter that is outside the range allowed by Chromeleon To troubleshoot Enter a valid setting for this parameter Cause The Volume entered for SyringeAspirate or SyringeDispense in the Comm...

Page 106: ...pecify both the destination for the sample to be dispensed and the volume of the syringe installed in the syringe pump Cause The Chromeleon program contains more than one PrepareNextSample command for...

Page 107: ...oot Enter a valid position number The AS HV supports both a 15 position sample rack and a 24 position sample rack Valid position numbers for the racks are R1 to R15 or R1 to R24 respectively Cause The...

Page 108: ...b In Chromeleon select SyringeReset in the Commands dialog box and click OK Cause This error usually occurs when the initialization sequence cannot be performed correctly To troubleshoot 1 Check for b...

Page 109: ...ace 4 Initialize the AS HV to verify that it is in a working state Turn off the power wait 10 seconds and turn on the power again 5 Send some commands from Chromeleon to the AS HV while the commands a...

Page 110: ...t Stop the Chromeleon server after 30 seconds restart the server If the message appears again contact Dionex for assistance Cause The syringe pump failed to initialize To troubleshoot 1 Check for tubi...

Page 111: ...essfully initialized it will not accept commands a Turn off the power wait 10 seconds and turn on the power again b In Chromeleon select SyringeReset in the Commands dialog box and click OK Cause Bloc...

Page 112: ...e expressed as four digits for example for 400 psi enter 0400 Cause Your AXP pump was not purchased from Dionex and therefore cannot communicate with Chromeleon To troubleshoot Contact Dionex if you w...

Page 113: ...ooting steps in Section 4 2 and Section 4 3 Cause Although the axis motor was commanded to move the sampling needle failed to reach the desired position To troubleshoot Follow the troubleshooting step...

Page 114: ...tted for the sampling arm movement has been exceeded This indicates a problem with the AS HV firmware To troubleshoot Contact Dionex for assistance Cause Chromeleon is unable to communicate with the A...

Page 115: ...in PC board error Contact Dionex 0 0 1 0 2 Program memory checksum error page 88 0 0 1 1 3 Configuration memory checksum error page 88 0 1 0 0 4 RAM test error page 90 0 1 0 1 5 Axis initialization er...

Page 116: ...AS HV Operator s Manual 108 Doc 065125 01 10 06...

Page 117: ...D stainless steel needle lined and sleeved with PTFE 064519 Sampling needle guide Sample Racks 064233 Sample rack 24 position for Nunclon sample flasks 064234 Sample rack 15 position for narrow mouth...

Page 118: ...32 to USB cable 064350 Relay cable for external pump Miscellaneous 064052 Trace analysis cover 064251 Spill tray 064520 Fixed Rinse Reservoir Kit 064486 Fixed rinse reservoir inlet line 2 5 mm 0 10 in...

Page 119: ...s 37 Manual user 14 Pull mode flow schematic 74 Push mode flow schematic 74 RS 232 cable 73 Service procedures 47 B Baud rate Default setting 8 78 Bottles Nalgene 6 109 Caps and septa 6 109 Filling wi...

Page 120: ...Connection to Chromeleon PC 10 78 Connection to relay controlled pump 16 I I O port 9 Connection to syringe pump 13 71 100 Important icon 3 Installation 61 109 L Liquid leaks 40 M Maintenance procedur...

Page 121: ...leon commands 19 Connection to I O port 16 Installation 77 Maintenance procedures 37 Relay cable 77 Service procedures 47 RESET switch 8 Rinse fluid Specifications 7 37 Rinse pump 1 7 Default speed 24...

Page 122: ...ge pump valve replacement 55 56 Syringe replacement 48 51 Spill tray 1 6 Installation 62 Standards rack 1 6 109 Installation 66 Syringe Liquid leaks 39 Replacement 48 51 Speed settings 27 30 Syringe p...

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