DNAPac PA200
Document No. 065036
Page 7 of 25
2.3
DNAPAC PA200 Column Operational Parameters
TABLE 2 Column Operational Parameters
pH = 4-10 (unrestricted eluents)
pH Range:
pH = 2.5- 4, and 10-12.5: Operation at these pH values require co-ion
concentration (e.g., Cl
-
or ClO
4
-
at high pH and Na
+
or NH
4
+
at low
pH) to be at least equimolar with hydroxide at high pH or H
+
at
low pH.
Temperature Limit:
< 85°C
Pressure Limit:
4,000 psi
Organic Solvent Limit:
100% Acetonitrile, or methanol, if required for cleaning.
Chaotrope Limit:
30% formamide, 6 M Urea.
Note:
Use of these chaotropes will increase back pressure, and
reduce column lifetime.
Typical Eluents:
High purity water (18 megohm-cm), sodium chloride, sodium
perchlorate, buffers, sodium acetate and sodium hydroxide.
Detergent
Compatibility:
Nonionic, cationic or zwitterionic detergents.
CAUTION:
Do not use anionic detergents. Anionic detergents will bind irreversibly to the column.