Dionex 062998, Product Manual, Page: 4 / 25

DNAPac PA200 Document No. 065036 Page 4 of 25
SECTION 1 - INTRODUCTION
1.1 DNAPac PA200
The DNAPac PA200 is a pellicular anion exchange column designed specifically to provide high-resolution
separations of single stranded nucleic acids. The DNAPac PA200 provides n, n-1 resolution over a wide range of
oligomer lengths and can perform separations under a variety of denaturing conditions:
•
High temperature, pH 8 or below
•
High pH (12.4) at 30° or below
Because of the unique pH stability of the packing material, elevated pH conditions can be used to optimize
selectivity for specific oligonucleotides.
The packing material inside the DNAPac PA200 is composed of 130 nm quaternary amine functionalized
MicroBeads™ bound to an 8 µm solvent compatible, non-porous substrate. The non-porous substrate design
provides rapid mass transport resulting in narrow high efficiency peaks. The low column capacity, typical of non-
porous packings, is avoided by agglomerating functionalized MicroBeads to the surface of the substrate particle,
resulting in higher loading capacity than is possible with conventional non-porous materials, and good durability.
This produces a column with oligonucleotide resolution superior to columns using 2 to 3 µm resins.
Resin Characteristics:
Particle Size: 8 µm
Pore Size: non porous
Cross-linking: 55%
Ion exchange capacity:
~40 µeq/column
Latex Characteristics:
Functional Group: quaternary ammonium ion
Latex Diameter: ~130 nm
Latex Cross-link : 5 %
Typical Operating Parameters:
pH range: 4-10 unrestricted eluents
2.5-4 and 10-12.5, (Operation at these pH values require co-ion concentration to be
at least equimolar with hydroxide at high pH or H
+
at low pH)
Temperature: ≤ 85°C
Pressure: 3,000 psi
Organic Solvent Limit: 100% acetonitrile or methanol for cleaning
Typical eluents:
High purity water (18.2 megohm-cm), sodium chloride, sodium perchlorate, buffers,
sodium acetate and sodium hydroxide
1.2 BioLC System (without Columns)
Table 1: System Components Recommended for DNA Analysis
Basic Gradient System Standard Gradient System
BioLC gradient pump, with degas BioLC gradient pump (degas recommended)
Chromatography oven with injection valve
and regulator assembly
Autosampler
Column Oven
Absorbance detector (D
2
lamp for UV) Absorbance detector (D
2
lamp for UV)
EO1 eluent organizers EO1 Eluent organizers