Diffinity Genomics RapidTip2 User Handbook Manual Download Page 6

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Page 6

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Product Specifications

Capacity: PCR Sample Size

50 ±5 microliters

dsDNA Recovery

up to 90%
(100bp to 10Kb)

Impurity Removal (dNTP,
primers, primer-dimers)

up to 90%
(less than 40–50 bp)

DNA Polymerase removal
(Taq, Hot-Start, High-
Fidelity)

up to 1:1000 dilution with
no measurable amplification
activity

Diffinity RapidTip2 tips are designed to remove dNTPs,
primers, primer-dimers, and DNA polymerase at typical PCR
concentrations.

Summary of Contents for RapidTip2

Page 1: ...otice For optimal purification results sharply tap the RapidTip2 package 2 3 times on a flat surface so that the particles drop to the bottom of the tips Version B March 2012 For PCR Purification with...

Page 2: ...y Control 5 Technical Assistance 5 Safety Information Disposal 5 Product Specifications 6 Introduction 7 Diffinity Technology Principle 8 Equipment to be Supplied by User 9 Guidelines Pipetting with t...

Page 3: ...ity RapidTip2 for PCR Purification with Polymerase Removal 1 Box of 48 RapidTip2s RR050 096 RR050 096L Diffinity RapidTip2 for PCR Purification with Polymerase Removal 1 Box of 96 RapidTip2s L denotes...

Page 4: ...asing compound Product Warranty and Satisfaction Guarantee Diffinity Genomics will guarantee the performance of Diffinity RapidTip2s for the uses and performance as described in our product literature...

Page 5: ...nce Call 1 877 362 1812 between 8am and 4pm Eastern Time Monday through Friday or visit www diffinitygenomics com for help with common questions Safety Information Disposal Diffinity RapidTip2s may be...

Page 6: ...0bp to 10Kb Impurity Removal dNTP primers primer dimers up to 90 less than 40 50 bp DNA Polymerase removal Taq Hot Start High Fidelity up to 1 1000 dilution with no measurable amplification activity D...

Page 7: ...emoves dNTPs primers primer dimers and DNA polymerase while providing up to 90 recovery of pure DNA fragments ranging in length from 100bp to 10Kb It dramatically reduces the amount of time to purify...

Page 8: ...urifies PCR solutions and removes DNA polymerase comparably to popular spin column methods The positive control con tains polymerase and all PCR reagents but did not undergo purification and therefore...

Page 9: ...how improved peak quality and lower background in Diffinity RapidTip2 purified samples as compared to unpurified samples Equipment to be Supplied by User PCR Sample 50 l 5 l Tubes to store purified DN...

Page 10: ...ate the rest of the sample and dispense 2 Mix effectively For effective mixing it is important that the particles remain in contact with the sample during each aspirate and dispense cycle Aspirate slo...

Page 11: ...mple gently flick the pipettor downward to bring them back into contact with the sample and proceed with mixing Pipetting Examples Please note that typical PCR samples are colorless for visualization...

Page 12: ...particles that purify a PCR reaction these particles can adhere to the pipette tip walls during shipping For optimal results sharply tap the box 2 3 times on a flat surface so that particles are at th...

Page 13: ...sary to drive liquid completely out of the tip on every dispense The particles should mix completely with the solution and make it appear cloudy while inside the tip 6 Dispense all solution into a cle...

Page 14: ...to load PCR mixes that contain density increasing compounds Fluid remains in the RapidTip2 It s normal for a small amount of liquid 10 l to remain inside the Diffinity RapidTip2 Note Overdispense or...

Page 15: ...dwide For a complete list visit our website 2 Call Diffinity Genomics Customer Service and place the order by phone 1 877 362 1812 3 Visit our website and place an order online via the online Diffinit...

Page 16: ...es Diffinity Genomics Inc 150 Lucius Gordon Drive West Henrietta NY 14586 2011 Diffinity Genomics Inc All rights reserved Diffinity Genomics Diffinity and RapidTip are trademarks of Diffinity Genomics...