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RapidTip2 PCR Purification Protocols
Prepare Samples:
Diffinity RapidTip2 is optimized for 50 μl reaction volumes but
can effectively purify samples from 45–55 μl.
• For PCR volumes >55 μl, please aliquot 50 μl into a new
tube for purification.
• For PCR volumes <45 μl, please dilute to 50 μl. This works
best with highly concentrated DNA samples (>50 ng/μl)
as your sample concentration will be reduced.
Prepare Tips:
Diffinity RapidTip2 for PCR Purification contains proprietary
particles that purify a PCR reaction; these particles can adhere
to the pipette tip walls during shipping. For optimal results,
sharply tap the box 2–3 times on a flat surface so that particles
are at the bottom of the tips (near the nozzle).
Please note that it is normal to see fine dust-like particles on the side of the
tip. After tapping the box, you should expect to see about 3mm of white
particles above the retainer at the small end of the pipette tip.
Purify Samples:
1. Program pipettor to aspirate
50 or 60 μl???
.
2. Place Diffinity RapidTip2 on pipettor — single or
multichannel.
Please note that you can use a multi-channel pipettor to mix more than
1 sample at a time for even higher productivity.
