RapidTip PCR Purification Protocols
Prepare Samples:
Diffinity RapidTip is optimized for 25 uL reaction
volumes but can effectively purify samples from 20-
30 uL.
• For PCR volumes > 30 uL, please aliquot 25
uL into a new tube for purification.
• For PCR volumes < 20 uL, please dilute to 25
uL. This works best with highly concentrated
DNA samples as your sample concentration
will be reduced.
Prepare tips:
Diffinity RapidTip contains proprietary particles that
purify a PCR reaction; these particles can adhere to
the pipette tip walls during shipping. For optimal
results, sharply tap the box 2-3 times on a flat
surface so that particles are at the bottom of the tips
(near the retainer).
Please note that it is normal to see fine dust-like particles on the
side of the tip. After you tap the box, you should expect to see
about 2mm of white particles above the retainer at the small end
of the pipette tip.
Purify Samples:
1. Program pipettor to aspirate 50 uL.
Diffinity RapidTip
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