Pipetting with the RapidTip
Careful pipetting is important to achieve effective
purification with the RapidTip. The following
pipetting guidelines will help ensure optimal results:
1. Aspirate slowly to ensure particles are dispersed
for effective mixing.
If using a manual pipettor
,
aspirate slowly for the
first several cycles until the particles are completely
dispersed into the PCR sample.
If using an automated pipettor, set to aspirate on
low speed (2 of 10 or equivalent), dispense on
middle speed (5 of 10 or equivalent) and place into
manual mix mode.
2. Maintain continuous contact between the
end of the pipette tip and your sample. Avoid
disrupting the fluid column with air bubbles.
3. Maintain clearance between the end of the
pipette tip and the plastic tube or well holding
sample to avoid plugging the tip during sample
mixing.
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