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Diffinity Genomics

RapidTip

TM

User Handbook

Version D (December 2010)

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Summary of Contents for RapidTip

Page 1: ...Diffinity Genomics RapidTipTM User Handbook Version D December 2010...

Page 2: ...Control 5 Technical Assistance 5 Safety Information Disposal 5 Product Specifications 6 Introduction 7 Diffinity Technology Principle 8 Equipment to be Supplied by User 9 Pipetting with the RapidTip 1...

Page 3: ...Purification 1 Box of 96 RapidTips RT025 096 Diffinity RapidTip for PCR Purification Multiple box purchases 3 12 24 and 48 are also available All Kits come with a User Manual Storage Diffinity RapidTi...

Page 4: ...antee Diffinity Genomics will guarantee the performance of Diffinity RapidTips for the uses and performance as described in our product literature If the product fails to perform to your satisfaction...

Page 5: ...hnical Assistance Call 1 877 362 1812 between 8am and 4pm Eastern time Monday through Friday or visit our webpage for help with common questions Safety Information Disposal Diffinity RapidTips may be...

Page 6: ...Impurity Removal dNTP primers primer dimers up to 90 less than 45 50 bp Please note that at this time the Diffinity RapidTip does not remove DNA polymerase Standard DNA polymerase such as Taq does not...

Page 7: ...y technology effectively removes dNTPs primers and primer dimers while providing up to 90 recovery of pure DNA fragments ranging in length from 100bp to 10Kb It dramatically reduces the amount of time...

Page 8: ...enters the pipette tip Dispensing the solution yields purified high quality DNA ready to use for downstream applications You won t need additional equipment reagents buffers or enzymes only a pipetto...

Page 9: ...ms show improved peak quality and lower background in Diffinity RapidTip purified samples as compared to unpurified samples Equipment to be Supplied by User PCR Sample Tubes to store purified DNA Pipe...

Page 10: ...he particles are completely dispersed into the PCR sample If using an automated pipettor set to aspirate on low speed 2 of 10 or equivalent dispense on middle speed 5 of 10 or equivalent and place int...

Page 11: ...are tips Diffinity RapidTip contains proprietary particles that purify a PCR reaction these particles can adhere to the pipette tip walls during shipping For optimal results sharply tap the box 2 3 ti...

Page 12: ...tip to begin the next mix It is not necessary to drive liquid completely out of the tip on every dispense The particles should mix completely with the solution and make it appear cloudy 4 Dispense al...

Page 13: ...and ready load PCR mixes that contain density increasing compounds Fluid remains in the RapidTip It is normal for a small amount of liquid 5 uL to remain inside the Diffinity RapidTip Note Overdispen...

Page 14: ...diffinitygenomics com or call 1 877 362 1812 c 2009 Diffinity Genomics Inc All rights reserved Diffinity Genomics Diffinity and RapidTip are trademarks of Diffinity Genomics Inc All other names may be...

Page 15: ...Notes Diffinity RapidTip Page 15...

Page 16: ...Diffinity Genomics RapidTip User Handbook Version D December 2010...

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