PROTOCOLS
Check
the speed
recommendations in
the manual* according to your protocol
(optimized protocols in the table below)
https://www.diagenode.com/en/p/megaruptor-3
Tab Documents
Set
parameters
(concentration, volume, and speed)
3
4
Or find your pre-recorded shearing protocol
on User protocols
PROTOCOLS
Standard
Low volume
High
concentration
Sample Concentration
0-50 ng/µl
51-150 ng/µl
Sample Volume
100-500 µl
65 µl
200-500 µl
Fragment Length Achievable 5-100 kb
12-75 kb
5-100 kb
If the conditions are different from the above table, further
optimization steps are needed. Please contact Diagenode