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Dear customer,

Thank you for choosing our device and for your confidence in Diagenode.

We have designed this device using our higest level of knowledge in shearing so that it exceeds your expectations. This 
device will serve your research needs for many years and will provide the best experience with long DNA molecules 
preparation.

Summary of Contents for Megaruptor 3

Page 1: ...Megaruptor 3 Sleek Innovative Unmatched Diagenode Shearing Machine ...

Page 2: ...fidence in Diagenode We have designed this device using our higest level of knowledge in shearing so that it exceeds your expectations This device will serve your research needs for many years and will provide the best experience with long DNA molecules preparation ...

Page 3: ...fore using your Megaruptor 3 to avoid the risk of personal injury and damage Do not use the Megaruptor 3 for anything other than its intended use After removing the packaging make sure that the Megaruptor 3 is intact Do not use the Megaruptor 3 in a cold room Do not allow the Megaruptor 3 to be used by untrained individuals Clean the Megaruptor 3 carefully and regularly with soft tissue and water ...

Page 4: ...eight 6 kg with Cassette included 0 8kg Fragment Length Range Achievable 5 kb 100 kb Sample Volume Range 65 500 µl Sample Concentration Range 0 150 ng µl Throughput 1 to 8 samples in parallel Time per Run Variable depending on sample volume concentration and target size Shearing Consumables Megaruptor 3 Shearing Kit 16 samples Cat No E07010003 User Interface Touchscreen with Megaruptor software ...

Page 5: ...Power Button Led Light Bar Touchscreen Lid Cassette Plug Removable Cassette Sample Insert Base unit ...

Page 6: ...cols Record your protocol Information General information about your Megaruptor 3 Settings Visualize and change the parameters of the Megaruptor 3 Initialization Set the system to its 0 position DESCRIPTION Protocols Run a shearing experiment follow instructions Advanced Mode Access for administrators Modification Modify the parameters ...

Page 7: ...of your samples The blue light means the system is waiting for the operator and is flashing if the system needs to be initialized The green light indicates the progression of the run The orange light means the protocol is on pause The red light indicates an alert message ...

Page 8: ...Start and initialize the device 1 2 INSTRUCTIONS The initialization step must be performed if the icon is flashing Start the shearing experiment ...

Page 9: ... 3 4 Or find your pre recorded shearing protocol on User protocols PROTOCOLS Standard Low volume High concentration Sample Concentration 0 50 ng µl 51 150 ng µl Sample Volume 100 500 µl 65 µl 200 500 µl Fragment Length Achievable 5 100 kb 12 75 kb 5 100 kb If the conditions are different from the above table further optimization steps are needed Please contact Diagenode https www diagenode com en ...

Page 10: ...Sample preparation between 65 500 µl Spin your sample s DNA solution in the 0 150 ng µl range 5 6 Hydro Tube INSTRUCTIONS ...

Page 11: ...ge 8 Insert the sample s in the cassette 1 to 8 samples Watch the video on our Diagenode Youtube channel How to set up your sample s Always set consumables with sample or empty at each extremity of the cassette Hydropore Syringe Hydro Tube Plug ...

Page 12: ...Let your Megaruptor running 9 Collect your sample s 10 INSTRUCTIONS ...

Page 13: ...If you have any question please contact the specialist at https www diagenode com en pages support ...

Page 14: ...ly unnecessary MANUAL SAFETY APPROVAL Megaruptor 3 has been assessed and certified under the standards ENVIRONMENTAL PROTECTION Packaging materials contain recyclable materials Read the full manual https www diagenode com en p megaruptor 3 Tab Documents ...

Page 15: ...purchaser of the product If a manufacturing defect arises and a valid claim is received within the warranty period Diagenode at its discretion will repair or replace the product in accordance with the warranty terms and conditions stated herein In case of repair or replacement of a product under warranty Diagenode will cover the expenses to return the repaired or replacement product This warranty ...

Page 16: ... 2019 Diagenode Inc All rights reserved The content of this document cannot be reproduced without prior permission of the authors ...

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