6.4.1
Calibration of the feed pump
Follow the instructions below to calibrate the system flow for the feed pump.
Step
Action
1
Block the retentate line to the tank by using the pinch clamp of the
R2L
tubing (1), and block the transfer line to the tank by adding a stopper (2) in
the tank to transfer port.
2
Fill the tank with water (3).
3
Replace the filter with tubing (4) between the feed pump pressure sensor
and retentate pressure sensor.
4
Open the upper drain valve to collect the flow (5) in beaker A.
5
Set a low rpm value of the feed pump on the control panel and tap
Start. Let
the pump run for a couple of minutes. Fill more water in the tank, if needed.
For an instruction how to use set-points, see
Adjust set-points, on page 161
.
6 Run preparations
6.4 Calibration
6.4.1 Calibration of the feed pump
110
ÄKTA flux s Operating Instructions 29046893 AG
