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On the Result screen
The following operations are performed on the result screen after counting.
After completing cell count, histograms of cell size distribution and result images are provided. While
viewing histogram, it is possible to modify cell size gating parameter. FACSCOPE
TM
B can generate
both histogram of Individual channel and combined histogram of all channels.
FACSCOPE B can detect 5 ~ 60µm objects in diameter. However, the gating system is set to
count from 8µm by default because the common cell line size is higher than 8µm.
Thus, the Initial range of cell size gating parameter on a histogram is automatically arranged
between 8µm and maximum size of the recognized cell population.
NOTE: If you want to count smaller than 8 µm, change the cell size gating parameter in the histogram.
Toggle between histogram and result image once select a channel.
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Summary of Contents for FACSCOPE B
Page 1: ...Automatic Cell Counter Instruction manual...
Page 37: ...37 STEP 3 Move both columns and adjust the cell size STEP 4 Check the results 19 12 26...
Page 39: ...39 STEP 3 Press Data icon STEP 4 Check the number of Live cell and Viability 60...
Page 46: ...46 F Showing ID Step 1 To see each of Channel IDs press Channel ID To go back press Back...
Page 47: ...47 On the Setting screen FN...
Page 55: ...55 Appendix B Examples of error and inaccurate result 1 Too Low error 2 Too High error...
Page 58: ...58 Appendix D Example and explanation of PDF report...
