23
B. Setting Count mode
FACSCOPE B provides three counting modes (Quick/Normal/Precise mode) according to counting
area. FACSCOPE B is designed to capture multi frames per a channel using XYZ stage. Single frame
captured by FACSCOPE
TM
B has volume of 0.15 µL. The more pictures are taken, higher the accuracy
of results.
Select the count mode depending on situation refer to the following table.
Count mode
the number of
frames captured
per a channel
Analyzed
volume
Counting
time
per a test
In the case
Quick mode
1
0.15µL
≤
20s
When you want to get a result quickly and
make a rough estimate of cell number.
Normal mode
(default)
6
0.9µL
≤
30s
When you want to get results with
reasonable accuracy and speed (such as
general subculture procedure)
Precise mode
24
3.6µL
≤
100s
When you want to get a precise result and
count cell at low concentration
NOTE: if the cell concentration is less than 5X10
4
cells/ml, Precise mode is recommended.
STEP 1.
Press the Normal (Count mode) button.
2
Summary of Contents for FACSCOPE B
Page 1: ...Automatic Cell Counter Instruction manual...
Page 37: ...37 STEP 3 Move both columns and adjust the cell size STEP 4 Check the results 19 12 26...
Page 39: ...39 STEP 3 Press Data icon STEP 4 Check the number of Live cell and Viability 60...
Page 46: ...46 F Showing ID Step 1 To see each of Channel IDs press Channel ID To go back press Back...
Page 47: ...47 On the Setting screen FN...
Page 55: ...55 Appendix B Examples of error and inaccurate result 1 Too Low error 2 Too High error...
Page 58: ...58 Appendix D Example and explanation of PDF report...
