1. Click , the Setup window opens, with the Thermal Protocol
tab visible.
2. Click / to add/delete segment.
3. Click / to add/delete step.
4. The camera icon indicates that the fluorescence is being read.
5. Click to add the Melt Curve profile.
6. Click to save Protocol
7. Click to choose and use the saved Protocol (*.pdt)
Set up the Thermal Profile
The "plate setup" involves the following steps:
1. Click , the Plate Setup window opens.
2. Click to choose the sample.
3. Set up sample name.
4. Set up sample
t
ype: unknown, positive control, negative control, standard.
When the sample type is defined as “Standard”
the concentration setting interface will be promoted for user to set the
“Standard” concentration in the following interfaces:
Define the Plate Layout
Define Sample Name
Define Sample Type
Define Fluorophore CH1(None/SYBR/FAM)
Define Fluorophore CH2(None/Texas Red/ROX)
Define the concentration unit for samples
Click to choose the sample(1-8)
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