Concepts
·
The weight of one genome (g) = (size of genome in bp) x (618 g/mol/
bp)/Avogadro’s number
One human genome (g) = (3x10
9
bp) x (618g/mol/bp) / (6.02x10
23
) =
3.08x10
-12
g
One haploid cell (sperm/egg) = 3.08 pg of DNA
One diploid cell = 6.16 pg of DNA
·
RNA concentration (μg/μl) = (A260×40×D)/1000, where D = dilution
factor and A260= absorbance at 260 nm.
·
DNA concentration (μg/μl) = (A260×50×D)/1000, where D = dilution
factor and A260= absorbance at 260 nm.
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