6
7
Setting the Volume
After filling the pipet, the end should be dried with an absorbent paper that will not leave
impurities, in order to remove any excess solution on the outside surface of the pipet.
To set the required liquid volume precisely, press the dispense button gently (Figure 3C),
dispense the excessive liquid from the pipet until the meniscus of the liquid aligns exactly
with the required volume mark on the pipet.
Emptying the Pipet
Holding the vessel in an angled position, place the pipet end in contact with the vessel
wall and press the dispense button gently (Figure 3C). The dispensing intensity may be
adjusted depending on pressure applied to the dispense button. The greater the pressure
applied, the faster the outflow of liquid from the pipet.
The pipet controller has two dispense modes. The dispense mode is selected with the
BLOW/GRAV switch (Figure 1D).
• Gravity mode marked with the icon on the display (Figure 2.4): Dispensing is effected
in gravity mode, which means that the liquid flows out of the pipet by its own weight.
• Blow out mode marked with the icon on the display (Figure 2.5): Dispensing is effected
in gravity mode, however, when the dispense button is pressed to the middle position,
the pump is started and fast emptying of the pipet with a blow out is effected.
CAUTION:
During gravimetric dispensing the pipet may not be completely emptied due
to the characteristics of pipets used with the pipet controller.
6. Troubleshooting
If during your work the pipet controller is not functioning correctly, check the cause and
correct the fault.
Problem
Possible cause
Action
The pipet falls out (the
holding force of the pipet
is too small), or tilts to the
side too much.
The pipet holder is dirty or wet
(Figure 1G).
Take out the pipet holder,
and clean, wash, and dry it.
The pipet holder is damaged.
Replace the pipet holder
with a new one.
The pump is working, but
the pipet controller aid does
not draw liquid or draws
liquid very slowly.
The filter is dirty (Figure 1H).
Take out the pipet holder, take out
the filter, if it is dirty, replace it
with a new one.
The pipet holder and/or the
connector gasket is damaged
(Figure 1J).
Replace the mechanically
damaged elements with new
ones.
Liquid leaks from the pipet
(the aspiration and the
dispense buttons are not
pressed)
The pipet is damaged.
Check the pipet for damage
(cracks, dents) if present, replace
the pipet with a new one.
The pipet is inserted
incorrectly.
Check whether the pipet has been
correctly inserted in the pipet
holder.
The pipet holder, the filter, or
the connector gasket is installed
incorrectly.
Check whether all parts are
present and correctly installed.
The pipet holder and/or the
connector gasket is damaged
(Figures 1G, 1J).
Replace the mechanically
damaged elements with new
ones.
If the above actions do not remedy the incorrect functioning of the pipet controller, the
device should be sent to the nearest authorized service center.
• The pipet controller should not be used for drawing solutions with a temperature above
50°C.
• The pipet controller should be operated in the temperature range from +10°C to +35°C.
• The pipet controller is suitable for general laboratory use only. It must be used only by
personnel who know the health risks associated with the substances that are normally
used with this instrument.
4. Turning On the Pipet Controller
The pipet controller is switched on by pressing the aspiration button (Figure 1A) or the
dispense button (Figure 1B). The display will show the selected aspiration mode, dispense
mode, and the battery level indicator (Figure 2.3). Examples of the display indications are
shown in Figure 5. The battery is discharged and requires charging if the indicator displays
one “bar” (Figure 5.3). When the battery is fully charged, the indicator displays three “bars”
(Figure 5.1). The pipet controller will switch off automatically if not used for 5 minutes.
The pipet controller should be charged only with the original charger.
The main voltage should conform with the specification on the charger.
Charging should be done in accordance with section 8 of this instruction for use.
5. Aspirating and Dispensing Liquids
Attaching a pipet
CAUTION
: Before attaching a pipet, check whether the pipet is not damaged, has no dents
or sharp edges in the gripping section. Check to ensure the gripping section is dry.
The pipet controller should be gripped as close to the upper end as possible and carefully
inserted into the pipet controller holder until resistance is felt (Figure 3A). Do not apply
excessive force, to prevent damage to thin pipets controller and to avoid injury risk. A pipet
that has been correctly attached and sealed in the holder should not tilt to the side.
After attaching a pipet, hold the device in such a way as to keep the pipet controller in
a vertical position with an attached pipet. When not in use, it is not recommended to leave
the pipet attached for long periods, for example overnight or over a weekend.
Note
: Do not rest the pipet controller on the bench if there is liquid in the pipet.
Filling the pipet
Before aspirating is started, set the suction speed switch using the HIGH/LOW switch
(Figure 1C):
• HIGH position: fast aspirating (Figure 2.1)
• LOW position: slow aspirating (Figure 2.2)
It is recommended to choose the LOW position for pipets with a volume up to 5 mL, and
the HIGH position for pipets with a volume greater than 5 mL. Holding the pipet controller
vertically, immerse the pipet end in the liquid to be drawn up (Figure 3B), and press the
aspiration button gently. The pipet filling speed depends on the pressure applied to the
aspirating button. The greater the pressure applied, the faster the liquid is aspirated into
the pipet.
It is recommended to draw a slightly larger liquid volume than required (due to meniscus
above the required volume mark), reducing the aspiration speed, particularly in the final
filling stage, so as not to overfill the pipet.
