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6.10
Calibrate Shock
This option is used to measure and calibrate the stimulus. It will switch ON the electric stimulus
continuously (instead of pulsed) to allow a stable reading from a connected ampmeter (RMS value typical).
The average current delivered to the animal is the continuous RMS current multiplied by the dutycycle of
the chosen repetition rate.
For reference, the stimulus pulse duration is 200ms. Likewise, the 1Hz, 2Hz and 3Hz periods are 1000ms,
500ms and 330ms respectively. Therfore, each the repetition rate’s dutycycles are:
o
1Hz:
0.20
o
2Hz:
0.40
o
3Hz:
0.61
For example, a measured RMS current of 1.0mA will deliver an average current of 200uA, 400uA and
610uA respecitvely.
To measure the the electric stimulus, you will need the following items:
o
AC ammeter (low range: 0-10mA).
o
Alligator test leads for the AC ammeter.
o
Alligator jumper wire.
o
Fixed external resistance (animal body equivalent, 10,000 ohms typical, if used).
Enable the stimulus for the mouse lane 1 or 6 buy setting the toggle switch on the treadmill toward the
lane (by default, the stimulus should not be present while navigating the User Menu).
Install the alligator test leads into the appropriate current-measuring terminals on the AC ammeter.
Switch ON the meter and select the range to measure no more than 10mA.
If using a fixed external resistor (animal body equivalent):
o
Connect the red meter lead to one terminal of fixed external resistor.
o
Using the alligator jumper wire, connect the free end of the external resistor to the fourth (4th) grid
in from the end of the grids (in lane 1 or 6).
If not using a fixed external resistor, connect the red meter lead directly to the fourth (4th) grid in from
the end of the grids (in lane 1 or 6).
Connect the black meter lead to the second (2nd) grid in from the end.
This completes a closed-circuit between the full-wave current source, animal body resistance and AC
ammeter.
Summary of Contents for Exer 3
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