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CEM liberty, User Manual

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Spare Parts

For additional parts visit our website: www.cem.com

Filters

Part Number Name

Description

551005-M

Dip Tube Filters

Replacement filters for all reagent dip tubes (bag of 30 filters)

551085

Vent Line Filter

Replacement filter for the vent line (located before RV1)

551000

External Bottle Filter Assembly

To be used for main solvent, deprotect, and transfer solvent bottles ONLY

Bottles and Caps

Part Number Name

Description

551320

250 mL Bottle

Safety coated glass 250 mL Bottle used for Activator/Base

551325

500 mL Bottle

Safety coated glass 500 mL Bottle used for Deprotection

551330

1 L Bottle

Safety coated glass 1 L Bottle used for wash solvent

177285

6 L Carboy

Plastic 6 L carboy used for waste

Temperature Probe

Part Number Name

Description

314325

Fiber Optic Probe

Probe used to measure and control reaction temperature

Reaction Vessel Selection

Part Number Name

Description

167260

30 mL Reaction Vessel

Standard reaction vessel used for 0.005 - 0.25 mmol syntheses (up to 1 g of resin).

542415

125 mL Reaction Vessel

Optional larger reaction vessel used for 0.5- 5 mmol syntheses (up to 8 g of resin).

Summary of Contents for liberty

Page 1: ...e is designed to highlight the basic chemistry and functions of the Liberty Blue Read and understand the Manual PN 600291 before operating the Liberty Blue For More Information Liberty Blue Operation Manual PN 600291 detailed information about setup and operation of the system CEM website www cem com list of spare parts pictures latest information about CEM s products references application notes ...

Page 2: ... 5 0 mmol Wash Solvent DMF DMF DMF DMF DMF DMF Deprotection Cocktail 4 Piperidine in DMF or 2 Piperazine w v in EtOH NMP 10 90 4 Piperidine in DMF or 2 Piperazine w v in EtOH NMP 10 90 20 Piperidine in DMF or 10 Piperazine w v in EtOH NMP 10 90 20 Piperidine in DMF or 10 Piperazine w v in EtOH NMP 10 90 20 Piperidine in DMF or 10 Piperazine w v in EtOH NMP 10 90 20 Piperidine in DMF or 10 Piperazi...

Page 3: ...re cleavage of the peptide from the resin low yields Activation methods containing DIEA are more basic and thus preferred to DIC Oxyma for these resins at high temperatures Since lower temperatures should be used the more aggressive HATU activator is recommended CEM Corporation offers Cl TCP Cl ProTide which shows excellent stability to high temperatures and acidic additives such as Oxyma Pure yet...

Page 4: ...istidine His Boc suppresses epimerization at elevated temperature and by default is coupled using the standard coupling microwave method His Trt is susceptible to epimerization at elevated temperature His Trt should be coupled using the single 50 C 10 min couplpling cycle to minimize epimerization using the parameters in the table below Stage Temp o C Power W Time sec Delta T 1 25 0 120 2 2 50 35 ...

Page 5: ...eavage of the peptide In these cases the use of Fmoc Asp OMpe OH in place of the more common Asp OtBu can reduce aspartimide formation without the addition of Oxyma Pure HOBt The Asp Gly DG segment is particularly sensitive to base catalyzed aspartimide formation Simple addition of HOBt or Oxyma Pure to the standard deprotection solutions may not be sufficient to prevent this side reaction The use...

Page 6: ...sses oxidation of Cys Met side chains Cleavage can be performed either at room or elevated temperatures The table below lists CEM s recommended parameters for both elevated and room temperature cleavage For sequences with more than 3 arginine residues extend cleaving time by 5 minutes per Arg after 3 if using heated cleavage or by 30 minutes if using room temperature cleavage Avoid cleaving for ov...

Page 7: ...positions Reaction Scale for Resin Transfer The resin transfer option is intended for syntheses at 0 1 or 0 25 mmol scale 0 25 1 0 g of resin The resin transfer option should only be used with the 30 mL reaction vessel This option has not been optimized by CEM for use with the 125 mL vessel Transfer Solvent The transfer solvent is used to move the resin into and out of the reaction vessel and to s...

Page 8: ...ty coated glass 250 mL Bottle used for Activator Base 551325 500 mL Bottle Safety coated glass 500 mL Bottle used for Deprotection 551330 1 L Bottle Safety coated glass 1 L Bottle used for wash solvent 177285 6 L Carboy Plastic 6 L carboy used for waste Temperature Probe Part Number Name Description 314325 Fiber Optic Probe Probe used to measure and control reaction temperature Reaction Vessel Sel...

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