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Liberty Blue

TM

Automated Microwave Peptide Synthesizer

User Guide

 

WARNING

 

Handle all chemicals under a fume hood, and wear suitable protective clothing such as safety glasses, chemical 
resistant gloves, and a laboratory coat. Dispose of all waste in accordance with all applicable local, state, and 
federal health and safety recommendations. For detailed information on the safety requirements for the chemicals 
used on the Liberty Blue, refer to the appropriate SDS documents.

This User Guide is designed to highlight the basic chemistry and functions of the Liberty Blue. Read and understand the 
Manual, (PN 600291) before operating the Liberty Blue.

For More Information

• Liberty Blue Operation Manual

 (PN 600291): detailed information about setup and operation of the system.

• CEM website

 (www.cem.com): list of spare parts, pictures, latest information about CEM’s products, references, 

application notes, and chemical reagents

• Applications Support

: contact CEM at (800) 726-3331 (US/Canada), (704) 821-7015, or by email at  

 

 

[email protected]

• Technical Support

: Contact CEM Service at (800) 726-5551 (US/Canada) or (704) 821-7015

Part Number 600329

November 11, 2018

Rev. 6

Copyright CEM Corporation 2018

Summary of Contents for liberty

Page 1: ...e is designed to highlight the basic chemistry and functions of the Liberty Blue Read and understand the Manual PN 600291 before operating the Liberty Blue For More Information Liberty Blue Operation Manual PN 600291 detailed information about setup and operation of the system CEM website www cem com list of spare parts pictures latest information about CEM s products references application notes ...

Page 2: ... 5 0 mmol Wash Solvent DMF DMF DMF DMF DMF DMF Deprotection Cocktail 4 Piperidine in DMF or 2 Piperazine w v in EtOH NMP 10 90 4 Piperidine in DMF or 2 Piperazine w v in EtOH NMP 10 90 20 Piperidine in DMF or 10 Piperazine w v in EtOH NMP 10 90 20 Piperidine in DMF or 10 Piperazine w v in EtOH NMP 10 90 20 Piperidine in DMF or 10 Piperazine w v in EtOH NMP 10 90 20 Piperidine in DMF or 10 Piperazi...

Page 3: ...re cleavage of the peptide from the resin low yields Activation methods containing DIEA are more basic and thus preferred to DIC Oxyma for these resins at high temperatures Since lower temperatures should be used the more aggressive HATU activator is recommended CEM Corporation offers Cl TCP Cl ProTide which shows excellent stability to high temperatures and acidic additives such as Oxyma Pure yet...

Page 4: ...istidine His Boc suppresses epimerization at elevated temperature and by default is coupled using the standard coupling microwave method His Trt is susceptible to epimerization at elevated temperature His Trt should be coupled using the single 50 C 10 min couplpling cycle to minimize epimerization using the parameters in the table below Stage Temp o C Power W Time sec Delta T 1 25 0 120 2 2 50 35 ...

Page 5: ...eavage of the peptide In these cases the use of Fmoc Asp OMpe OH in place of the more common Asp OtBu can reduce aspartimide formation without the addition of Oxyma Pure HOBt The Asp Gly DG segment is particularly sensitive to base catalyzed aspartimide formation Simple addition of HOBt or Oxyma Pure to the standard deprotection solutions may not be sufficient to prevent this side reaction The use...

Page 6: ...sses oxidation of Cys Met side chains Cleavage can be performed either at room or elevated temperatures The table below lists CEM s recommended parameters for both elevated and room temperature cleavage For sequences with more than 3 arginine residues extend cleaving time by 5 minutes per Arg after 3 if using heated cleavage or by 30 minutes if using room temperature cleavage Avoid cleaving for ov...

Page 7: ...positions Reaction Scale for Resin Transfer The resin transfer option is intended for syntheses at 0 1 or 0 25 mmol scale 0 25 1 0 g of resin The resin transfer option should only be used with the 30 mL reaction vessel This option has not been optimized by CEM for use with the 125 mL vessel Transfer Solvent The transfer solvent is used to move the resin into and out of the reaction vessel and to s...

Page 8: ...ty coated glass 250 mL Bottle used for Activator Base 551325 500 mL Bottle Safety coated glass 500 mL Bottle used for Deprotection 551330 1 L Bottle Safety coated glass 1 L Bottle used for wash solvent 177285 6 L Carboy Plastic 6 L carboy used for waste Temperature Probe Part Number Name Description 314325 Fiber Optic Probe Probe used to measure and control reaction temperature Reaction Vessel Sel...

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