CEM Discover SPS Installation Instructions Download Page 4

Innovators in 
Microwave Chemistry

 

 

CEM Corporation  

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  PO Box 200  

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  Matthews, NC 28106  

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  (800) 726-3331  

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  (704) 821-7015  

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  www.cempeptides.com 

 
 
 
 
 
 
 
 
 

5.  Recommended Parameters for Fmoc Solid Phase Synthesis 
 

a. Fmoc 

Deprotection 

Power = 20W* (Depends on sample size. Want to reach maximum temperature in 75 seconds. Adjust as 
necessary). 
Temperature = 75˚C* 
Time = 3:00 
Delta Temp. = 5˚C 
  

b. Coupling 

Power = 20W* (Depends on sample size. Want to reach maximum temperature in 75 seconds. Adjust as 
necessary). 
Temperature = 75˚C* 
Time = 5:00 
Delta Temp. = 5˚C 
Activation = HBTU or PyBOP w/ DIEA 
 

c. TFA 

Cleavage** 

Power = 20W(Depends on sample size. Want to reach maximum temperature in 120 seconds. Adjust as 
necessary). 
Temperature = 38˚C*** 
Time = 18:00 
Delta Temp. = 5˚C 
 
*An overshoot in temperature of 5C will be observed because of a delay in the fiber-optic probe signal 
due to the thermowell.  
**Caution certain scavengers (EDT, Thioanisole) can be very odorous. Ensure proper ventilation at all 
times.  
***Ensure that the cleavage temperature does not exceed 43˚C at any time 
 
 

 
 
 
 
 
 
 

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