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Innovators in
Microwave Chemistry
CEM Corporation
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PO Box 200
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Matthews, NC 28106
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(800) 726-3331
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(704) 821-7015
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www.cempeptides.com
5. Recommended Parameters for Fmoc Solid Phase Synthesis
a. Fmoc
Deprotection
Power = 20W* (Depends on sample size. Want to reach maximum temperature in 75 seconds. Adjust as
necessary).
Temperature = 75˚C*
Time = 3:00
Delta Temp. = 5˚C
b. Coupling
Power = 20W* (Depends on sample size. Want to reach maximum temperature in 75 seconds. Adjust as
necessary).
Temperature = 75˚C*
Time = 5:00
Delta Temp. = 5˚C
Activation = HBTU or PyBOP w/ DIEA
c. TFA
Cleavage**
Power = 20W(Depends on sample size. Want to reach maximum temperature in 120 seconds. Adjust as
necessary).
Temperature = 38˚C***
Time = 18:00
Delta Temp. = 5˚C
*An overshoot in temperature of 5C will be observed because of a delay in the fiber-optic probe signal
due to the thermowell.
**Caution certain scavengers (EDT, Thioanisole) can be very odorous. Ensure proper ventilation at all
times.
***Ensure that the cleavage temperature does not exceed 43˚C at any time