13
Use a small dry ice scoop or dry ice shovel to fill the
dry ice container up so that the polystyrene lid can still be
easily placed on top of the dry ice. Do not squeeze the
polystyrene lid in too far to avoid issues in retrieving.
Th
e lid for the dry ice container needs to be
securely tightened to reduce the risk of a
leakage into the incubation chamber
The container is designed to hold approximately 0,5kg of
dry ice pellets / nuggets with a diameter of 10mm. Avoid
overfilling the container to ensure that the polystyrene lid
can be replaced and the dry ice lid can be tightened se-
curely at least to its minimum marking.
Tap the CO
2
dry ice timer window in the Cellbox
Homescreen and reset the CO
2
dry ice timer, as de-
scribed in section 5.4.
A completely filled CO
2
dry ice container can sustain a
5% CO
2
concentration in the incubation chamber for at
least 24 hours.
If the incubation chamber is frequently opened or left
open for long periods during operation, there is a risk that
the dry ice may be depleted prematurely.
It is recommended to refill the dry ice container
and start the timer shortly before transport.
6.3
Preparing the device for transport
•
Switch on the Cellbox.
•
Verify the battery status and if necessary, charge the
device (observe the status of the 4 LEDs on the front
panel). Please keep in mind that 10
hours will be needed to fully charge the batteries.
•
If necessary, refill dry ice before use as described in
section 6.2.
•
It is essential to provide the device with sufficient
time to reach a stable internal temperature before
being used for the transport of living cells. The lid of
the incubation chamber has to be closed and
latched down before the device is switched on. Ap-
proximately 30-45 minutes are required for the inter-
nal temperature to reach 37°C.
•
The device can reach an internal CO
2
concentration
of 5% within a few minutes of operation.
All preparations should be performed
using an external power source to
ensure that the battery packs remain
fully charged for transport.
6.4
Transporting the device
The lid of the main power switch should be securely
closed before the device is transported. This prevents
access to the main power switch, ensuring the device is
not accidentally powered off during transport.
To protect the contents of the Cellbox, the device should
only be transported in an upright position.
It is strongly recommended that the Cellbox, and more
so the incubation chamber, remains closed during
transport. The incubation chamber should only be
opened if absolutely necessary because this jeopardizes
the internal environment and subsequently the cell viabil-
ity.
If the possibility exists, the device should be connected
to an external power source during transport. Dry ice can
also be refilled as desired. In this way the maximum
transport period of 24 hours can be extended.
