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4. 

Adjust the vertical position of the reflected image  

 

 

using V1 to centre the image.  The vertical field aper- 

 

 

ture size can be adjusted at this point to optimise the  

 

 

sensor use.

 

5. 

Adjust the vertical height of the transmitted image  

 

 

using V3 to centre.

 

6. 

Adjust the horizontal position of the transmitted  

 

 

image using H3 so the outer edge of the image is just  

 

 

inside the sensor edge.  If the images overlap, or there  

 

 

is a large gap between them, then adjust the horizon- 

 

 

tal field aperture size to optimise the sensor use, then  

 

 

readjustment of the split control will allow the outer  

 

 

edges of the images to be repositioned.

 

3.5 

Locating the OptoSplit images on the camera

If the OptoSplit III controls have been badly misaligned, the images can 

show significant aberrations, or in extreme cases only a single image 

may be observable for any setting of the ‘Split’ control. In these cases 

the following procedure will enable the device setup to be reconfig-

ured.

Locating the Reflected Image

 

1. 

Fit the system calibration cube & shutter the transmit- 

 

 

ted and centre illumination pathways using the shutter  

 

 

plates provided. 

 

2. 

Define a small area using the OptoSplit input aperture  

 

 

diaphragm and ensure the input to the OptoSplit is  

 

 

illuminated.

 

3. 

Attempt to centre the reflected image pathway  

 

 

horizontally using the ‘Split’ control to scan the   

 

 

position range.

 

4. 

If  no aperture edges can be located for any position  

 

 

of the ‘Split’control, enlarge the input aperture and  

 

 

repeat step 3.

 

5. 

Once the image of the input aperture has been  

 

 

located and centred horizontally, the vertical align- 

 

 

ment of the aperture can be set using the V1 control.

 

6. 

Adjust the input aperture size so that it just underfills  

 

 

the camera field of view vertically, and just under one  

 

 

third of the camera field horizontally.

Locating the Transmitted Image

 

1. 

Move the shutter from the transmitted to the    

 

 

reflected illumination pathway, the calibration cube 

 

 

should remain in place.

 

2. 

With the reflected image located, it is likely the   

 

 

transmitted image will be seen somewhere in the field  

 

 

of view. If this is the case, small adjustments of the H3  

 

 

and V3 controls should be used to adjust this image to  

 

 

mirror the location of the reflected image on the  

 

 

camera chip.

 

3. 

If no image is seen in the field, we will again scan the  

 

 

image horizontally. For the transmitted image this is  

 

 

achieved using the H3 control.

 

4. 

If after scanning the horizontal axis no image can be  

 

 

found, again open the input aperture slightly & repeat  

 

 

step 3.

 

5. 

Once the edges of the aperture have been found,  

 

 

reduce the input aperture as for step 6 above, and  

 

 

centre the image in the camera field using H3 and V3

Summary of Contents for OptoSplit III

Page 1: ...www cairn research co uk Free Phone 08453301267 UK only Tel 44 0 1795590140 Fax 44 0 1795594510...

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Page 4: ...rt department who are always willing to help e mail tech cairn research co uk Note Please do not adjust any control before reading this manual The OptoSplit is supplied pre aligned and centred please...

Page 5: ...plit III in the Lightpath Operation of the OptoSplit III Adjusting and Locking the Aperture Adjusting the Position of the Images Focussing the Camera on the Aperture Dual and Single Image Modes of Use...

Page 6: ...hin the camera frame Images can be acquired using any imaging software and processed either manually off line or using an appropriate analysis tool such as the Splitview module in Universal Imaging s...

Page 7: ...ces for three 25mm filters These are held in place by locking rings which can be removed using the tool provided To fit a filter remove the locking ring and place the filter orientated so that the arr...

Page 8: ...ular recess es Then simply replace the holder and reassemble the cube and tighten the screws 2 3 Inserting the Cairn Filter Cube into the OptoSplit III The Cairn filter cube is designed to be easily a...

Page 9: ...Page 4 Remove the access panel using the two handles Mounting bracket Replace the access panel using the two handles Mount the filter cube onto the brackect on the interior of the OptoSplit II...

Page 10: ...lear image can be seen the camera should be switched off and removed from the microscope The OptoSplit III should then be fitted on to the microscope with the diaphragm orientated toward the output of...

Page 11: ...cus The image should line up with the edges of the aperture and should not have any rotation or slant in either direction 3 Operation of the OptoSplit III The OptoSplit III uses a single control for a...

Page 12: ...e overlaid It is possible to separate the images by rotating the Split control in the clockwise sense however image distortion will be evident when overlaying the two halves of the image The imaging p...

Page 13: ...ontrol anti clockwise will seperate the reflected and transmit ted images symmetrically on the horizontal axis whilst the Centre image will not deviate Note To prevent calibration of the OptoSplit III...

Page 14: ...ong the vertical axis and allow for adjustments to be made independently of the Reflected image Adjustments to the H3 controller will move the Transmitted image along the horizontal axis Adjustments t...

Page 15: ...ken the two hex screws that retain the output rotating ring to allow the camera to turn in the C mount 3 Slightly loosen the single hex screw which locks the focussing ring 4 Rotate the focussing ring...

Page 16: ...erture Single image or bypass mode is the simplest of these only requiring the field diaphragm to be fully opened to enable operation in full frame mode When using the OptoSplit III with the regular O...

Page 17: ...e size to optimise the sensor use then readjustment of the split control will allow the outer edges of the images to be repositioned 3 5 Locating the OptoSplit images on the camera If the OptoSplit II...

Page 18: ...ra field horizontally Locating the Transmitted Image 1 Move the shutter from the transmitted to the reflected illumination pathway the calibration cube should remain in place 2 With the reflected imag...

Page 19: ...ontrol 4 If after scanning the horizontal axis no image can be found again open the input aperture slightly repeat step 3 5 Once the edges of the aperture have been found reduce the input aperture as...

Page 20: ...he slider is moved too far then the vignetting will become apparent on the other image and will need to be adjusted in the opposite direction until both images are the same intensity It is important t...

Page 21: ...e to give precise symmetrical control of the degree of separation whilst maintaining identical path lengths A half size fully silvered mirror at the output port for recombining the images Fine adjustm...

Page 22: ...age 17 5 Technical Support E mail tech cairn research co uk Web http www cairn research co uk Address Cairn Research Graveney Road Faversham Kent ME13 8UP Telephone 44 0 1795590140 Fax 44 0 1795594510...

Page 23: ...Page 18 In addition to designing and manufacturing our own products we are also proud distributors for the following companies...

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