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Introduction
In fluorescence imaging applications it is often useful to acquire simul-
taneous images at two emission wavelengths. Traditionally such appli-
cations have been restricted by the speed of an electronic filter
changer, or by the cost and complexity of adding a second camera to a
system. In many instances the region of interest (ROI) does not require
the full resolution of the camera, so the ideal solution would be to
simultaneously image at two different wavelengths on the same
camera chip. In conjunction with a research microscope and a suitable
CCD camera the Cairn Optosplit II allows the researcher to do exactly
this.
The Cairn Optosplit II is usually supplied with a rectangular aperture to
define a ROI and includes controls to allow the two images to be
positioned accurately and conveniently within the camera frame.
Images can be acquired using any imaging software and processed
either manually off-line or using an appropriate analysis tool such as
the Splitview module in Universal Imaging’s Meta series software or
Field Split in Andor Bioimaging’s iQ.
The instrument is usually configured to attach to the c-mount output
port of a research microscope, with a c-mount CCD camera fitted to its
output. The design allows for connection to a variety of alternative
devices, so please consult with us if you intend using it in any other
configuration.
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