Cairn OPTOSPLIT II BYPASS Troubleshooting Manual Download Page 2

Page: 2 / 13

+44(0)1795 590140 www.cairn-research.co.uk

1.2) Support legs

Two support legs are supplied for use with an inverted microscope to support the weight of the
unit.

1. Secure the

support leg bar

to the underside of the OptoSplit using the two screws provided

2. Screw the

support legs

to the end of the bar

3. The height can be adjusted on each support leg – we recommend a spirit level is used for final

adjustment.

2) Key controls

This section details the key controls for all operating modes – all labels are referred to in
subsequent text in bold blue font.

PASS

SPLIT

OPTOSPLIT

cairn-research.co.uk

Bypass

Zyla

Support leg bar

Support legs

Trim adjustment and clamp screw

Top face of camera

Summary of Contents for OPTOSPLIT II BYPASS

Page 1: ...uneven illumination which is often not introduced by the image splitter Once both your transmitted and fluorescent light source have been correctly aligned and focussed the OptoSplit can be mounted o...

Page 2: ...OptoSplit using the two screws provided 2 Screw the support legs to the end of the bar 3 The height can be adjusted on each support leg we recommend a spirit level is used for final adjustment 2 Key...

Page 3: ...arch co uk 2 1 OptoSplit panels B Left side panel A Top Panel BYPASS SPLIT OPTOSPLIT cairn research co uk Bypass Bypass split lever XY controls for Bypass image Split control Rectangular aperture adju...

Page 4: ...d to ensure the Bypass image is projected onto the centre of the camera sensor It s best practise to ensure this is carried out prior to performing dual channel imaging but this adjustment can be chec...

Page 5: ...door cover 3 Adjust the two rectangular aperture adjusters one horizontal the other vertical to create a single rectangular region of interest visible on your camera 4 Ensure the image is square with...

Page 6: ...imaging software is useful as a reference tool to ensure the image is centred and square 4 Obtaining a split image The predominant operating mode of two identical images projected onto each half of y...

Page 7: ...nd horizontally using the provided 1 5mm allen key on the H V controls on the magnetic door cover Once you are aware which channel is which turn your attention to the transmitted image and position th...

Page 8: ...l ensure maximal field of view is projected onto each half of your camera sensor Vignetting may be present once split mode has been optimised This is characterised by one image appearing darker on one...

Page 9: ...end the Chroma Ultra Flat UF 2 2mm thick dichroic mirror range The Cairn Filter Cube accepts dichroic mirrors of the following size 26mm x 38mm x 2mm Other dichroic mirrors will fit but may not be opt...

Page 10: ...3 Remove the blank cover for the auxiliary drop in position reflected path Note the blank hold ers will have no angled thread indictor and act as a protector for this pocket 4 Insert the rotating pola...

Page 11: ...ugins folder in your root MicroManager folder on the C drive A MicroManager plugin for use with version 2 0 is also currently in development Keep an eye on our website for more details Please contact...

Page 12: ...ange your microscope cube to a position suitable for Cy5 imaging 2 The mCherry emission filter will need to be removed as it will impair the Cy5 channel For ease of removal we recommend mounting the m...

Page 13: ...f required refer to section 3 2 5 Open the rectangular aperture To return to Split conditions 6 Slide the Bypass Split lever to Split 7 Replace the Cairn filter cube 8 Return your microscope cube to a...