Offline Operation
50
Scanning Probe Microscope Training Notebook
Rev. F
Figure 18.3c
Cross sections on first order flattened image of cells on a smooth glass substrate showing how
flattening can cause the distortion of the plane next to a raised feature in the fast direction
This problem can be remedied by excluding the raised or depressed features from the flatten
calculation by drawing boxes around them with the cursor before executing the flatten.
Figure 18.3d
Flattening an AFM Image of Cells on a Smooth Glass Substrate Using the Boxes to Exclude the
Cells from the Flattening Calculation
Planefit Auto
Planefit is commonly used to remove tilt or bow from images.
Planefit calculates a single polynomial fit for the entire image and then subtracts the polynomial fit
from the image.
Planefit may be applied by calculating a 1st, 2nd, or 3rd order polynomial fit to the image in the X
or Y directions.
•
1st order planefit removes tilt
•
2nd order planefit removes tilt and an “arch-shaped” bow
•
3rd order planefit removes tilt and an “S-shaped” bow
Image after 1st Order flatten
A
B
A'
B'
A-A'
B-B'
Use boxes to exclude raised features
from the flatten calculation
Image after 1st order flatten with raised
areas excluded from calculation
