4. Operation
CryoPod Carrier
Manual Filling of LN
₂
Part Number: 257437 Rev. A
Step
Action
4.
Position the liquid level sensor into the back interior corner diagonally opposite the funnel
assembly with the notch on the sensor touching the corner cut edge of the internal basket..
NOTE:
The rod must be in the lowest position.
5.
Fill the Dewar flask (not provided) with approximately 3 Liters of liquid nitrogen (not
provided).
6.
Slowly pour approximately one liter of LN
₂
into the funnel. Wait for the LN
₂
to stop boiling.
NOTE:
The funnel should never be more than half full at any time.
7.
Turn on the unit by short pressing
.
8.
Wait approximately 15 minutes until the temperature reaches -15°C. The LCD screen will
reflect the cooling chamber temperature.
NOTE:
If the CryoPod Carrier warms up over -20°C, the electronics will turn off
automatically after 1 minute.
9.
Slowly pour the remaining LN
₂
from the Dewar Flask into the funnel. Stop filling when the
sensor rod stops bouncing and the bottom of the colored fill bar is visible.
NOTE:
The funnel should never be more than half full at all times.
10.
Remove the funnel and fill sensor using cryogenic gloves. Place the lid back onto the unit.
Allow 10-15 minutes for the chamber temperature to equilibrate. When the CryoPod Carrier
Chamber reaches -170°C or below, the unit is ready for use.
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Copyright © 2016, Brooks Automation, Inc.
