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PHERAstar 

FS 

Operating Manual 

 

BMG LABTECH 

16/26 0471B0001A 

2009-09-22 

 

4.5.3  Time-Resolved Fluorescence Optic Module, FRET and TR-FRET, for example HTRF

®

 

The time-resolved fluorescence optic module (figure 20) is optimized for HTRF

®

. The beam splitters, dichoic 

mirror, as well as the filters, are all specially selected to give the very best performance. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

 

Figure 20: Optic module for time-resolved  

   fluorescence, 

for 

example 

HTRF

®

 

 
 

4.5.4  Luminescence Optic Modules 

Luminescence assays are mostly towards light of unspecific wavelength and therefore the default optic 
module is an empty module. The empty module (figure 21) still needs to be there because it limits reflection 
and scatter. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Figure 21: Optic module for luminescence, wavelength not specified. 
The LUM+ Module is intended for microplate formats up to 384 wells.  

 

Summary of Contents for PHERAstar FS

Page 1: ...Operating Manual Revision A...

Page 2: ...this manual Suggestions for improving this manual will be gratefully accepted BMG LABTECH reserves the right to change or update this manual at any time The revision number is stated at the bottom of...

Page 3: ...lications for fluorescence intensity fluorescence polarization time resolved fluorescence HTRF luminescence AlphaScreen and full spectrum absorbance The PHERAstar FS has high end performance in all pl...

Page 4: ...2 PLATE CARRIER IN OUT BUTTON 12 4 3 PHERASTAR FS SHIPMENT BOX 12 4 4 INSTALLING AND CHANGING OPTIC MODULES 13 4 4 1 BUILT IN BARCODE READER FOR OPTIC MODULES 14 4 5 DESCRIPTION OF OPTIC MODULES 15 4...

Page 5: ...g modes automatic gain selection manual gain available Optic modules Up to six application specific optic modules built in Additional optic modules available Spectral Range 230 nm 750 nm for FI FP LUM...

Page 6: ...nal type Wickmann only Dimensions and weight Height 46 cm width 46 cm and depth 51 cm Weight 50 kg max Ambient conditions Operating temperature 15 C to 35 C Storage temperature 10 C to 50 C Humidity o...

Page 7: ...3 Right side of the PHERAstar FS showing the reagent injection system Press the button on the grip and the optic module door can be slid aside Service lid for laser cartridge Plate carrier in out but...

Page 8: ...RAstar FS Figure 4 Back of PHERAstar FS Figure 5 Back of PHERAstar FS Main power on off switch Fan Air inlet Miscellaneous labeling such as serial Number CE marking and voltage information Main power...

Page 9: ...correct operation of the instrument and the safety issues Throughout this manual the word you refers to this trained operator Upon unpacking and positioning the reader make sure to unlock the transpo...

Page 10: ...ight and must not be moving as it was in the unlocked position Figure 7 Locking the plate carrier Never open the plate carrier door during measuring 3 1 3 Unlocking and Removing the Optic Module Trans...

Page 11: ...t the power switch on the back of the instrument is in the OFF position Inspect the voltage information on the label next to the power switch see figure 4 to ensure that it corresponds to the local ma...

Page 12: ...because the amount of air needed to maintain an acceptable inner environment can be acquired more easily through an open plate carrier door 4 2 Plate Carrier In Out Button The plate carrier in out bu...

Page 13: ...t view and side view When the PHERAstar FS is in its permanent position and powered ON the optic modules can be installed The PHERAstar FS has five positions for optic modules Once the optic module do...

Page 14: ...r inter changeable type a message will appear informing you manually to type in wavelengths in the optic module table The barcode detection will be triggered by a opening and closing the optic module...

Page 15: ...tested for optimal performance 4 5 1 Fluorescence Intensity and TRF Optic Module Standard fluorescence intensity optic modules are available in an almost unlimited variety of different configurations...

Page 16: ...y selected to give the very best performance Figure 20 Optic module for time resolved fluorescence for example HTRF 4 5 4 Luminescence Optic Modules Luminescence assays are mostly towards light of uns...

Page 17: ...ule to measure light of a specific wavelength Right luminescence optic module to measure light of two specific wavelengths 4 5 5 AlphaScreen Module AlphaScreen is a unique assay technology that measur...

Page 18: ...priming Refer to chapter 4 6 5 for a detailed description of the priming procedure 4 6 1 Reagent Injectors In order to retain optimal performance it is recommended to follow these guidelines Do not us...

Page 19: ...are assembled together in a magnetic clamp to minimize dead volume and to guide them to the needle holder The magnetic clamp can easily be removed by gently pulling it away from the instrument In orde...

Page 20: ...aste container and the needle holder are installed figure 28 The waste container must be placed underneath the needle holder and holds a total volume of max 15 ml Once 15 ml solution volume has been r...

Page 21: ...g position Hold on to the needle holder and place an external waste container beaker underneath the needle Priming is then initiated either via the Control Software or the respective priming buttons b...

Page 22: ...RAstar FS Connection to PC whereas the male to female RS232 cable included with the Stacker will connect only the PHERAstar FS to the Stacker If a Stacker is connected with a cable it will automatical...

Page 23: ...rier in all directions figure 31 It is locked if the plate carrier cannot be moved If the plate carrier can be moved and hence is not locked then either follow the described procedures again or lock i...

Page 24: ...same fluorescein concentration is an indication of incorrect or inaccurate dimensions of the microplate definition Contact BMG LABTECH service personnel for procedures Figure 33 The various reader off...

Page 25: ...or the instrument may not be accepted by the service centre or by customs authorities Use suitable disinfectants e g Alcohol 70 Authorized personnel wearing disposable gloves and protective glasses an...

Page 26: ...been disinfected according to the instructions given in the Operating Manual Name ________________________________________________ Firm ________________________________________________ ______________...

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