Biotek Synergy HTX, Operator'S Manual

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Chapter 3: Getting Started
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The Synergy HTX has two lamps: one for standard fluorescence, one
for absorbance and time-resolved fluorescence:
Standard Fluorescence: The 20-watt tungsten halogen lamp’s life is
rated at an average of 1000 hours, and it is user-replaceable. The
intensity of the bulb will slowly drop over time until the instrument’s
run-time self-check detects a low lamp current signal and Gen5
displays an error message. The lamp (PN 7080500) should be replaced
at this time. Keeping a spare lamp on hand is recommended.
Absorbance and Time-Resolved Fluorescence: This bulb should
outlive the useful life of the reader. If there is a problem with the
lamp, however, the intensity may drop and the run-time self-check will
detect a low signal level and generate an error message. If this
happens, the instrument will require service. Contact BioTek for
assistance (this lamp is not user-replaceable).
Excitation and Emission Filter Wheels
Synergy HTX models with fluorescence capability are equipped with one excitation
filter wheel and one emission filter wheel; readers with luminescence capability use an
emission filter wheel only. (A monochromator is used for absorbance measurements.)
A filter in the excitation wheel selects the narrow band of light to which the sample
will be exposed. A filter in the emission wheel selects the band of light with the
maximum fluorescence signal, to be measured by the photomultiplier (PMT).
Each filter wheel is labeled EX or EM, and can contain up to four filters and/or black
“plugs.” A filter can be used in either wheel, but it must be oriented properly, as
described below. Each filter and plug is held securely in place with a C-clip filter
retainer.

Each filter has its wavelength and bandpass values printed on its side,
with an arrow to indicate the proper direction of light through the
filter.

We recommend placing filters in the wheels in ascending wavelength
order from position 1 to 4 (no holes in EX2 or EM3), particularly if the
reader has generated a 4E18 (saturation) error.
BioTek Instruments, Inc.