Biotek Synergy H1, Operator'S Manual

Page: 145 / 190

Luminescence Test
Luminescence Testing starting on page 83 describes the test method, lists the Gen5
protocol parameters, explains how to analyze the test results, and provides
troubleshooting tips in the event of test failure.
Requirements
To perform this test, you will need:
l
Harta Luminometer Reference Microplate, BioTek #8030015 (which includes
microplate carrier adapter BTI #8042263)
l
Gen5 protocols, described on page 83 :
o
Synergy H1 F-LumTest_Harta (filter-based system)
o
Synergy H1 M-LumTest_Harta (monochromator-based system)
l
For use only with the filter-based test, the following filter set defined in Gen5:
Filter Set Name: Open
Excitation: Plug
Mirror: <none>
Emission: Hole
Test Procedure
1. Turn on the Harta reference plate using the I/O switch on the back of the plate.
2. Check the battery by pressing the test button on the back of the plate and ensuring
that the test light turns on. The test light may be difficult to see in bright light;
change your angle of view or move to a darker environment if necessary. If the light
does not turn on, replace the battery.
3. Place the adapter on the reader's microplate carrier and then place the Harta
reference plate on top of the adapter.
4. In Gen5, create an experiment based on the Synergy H1 F-Lum-Test (filter-based)
protocol and initiate a plate read.
The experiment begins with a three-minute Delay step.
5. When the experiment is complete, calculate and evaluate results as described under
Results Analysis on page 85 .
6. Repeat steps 4 and 5 using the Synergy H1 M-LumTest_Harta (monochromator-
based) protocol.
7. When finished, turn off the Harta reference plate to preserve battery life.
Synergy H1 Operator's Manual
Luminescence Test | 119