Biotek Synergy H1, Operator'S Manual

Page: 111 / 190

Synergy H1 M-LumTest_Harta.prt
The same as the filter-based test, with these exceptions:
Parameter Setting
READ STEP 1
Optics Type Luminescence Fiber
Filter Set <none>
Gain 150
Read Height 1.00 mm
READ STEP 2
Optics Type Luminescence Fiber
Read wells F1-G12
Filter Set <none>
Gain 150
Read Height 1.00 mm
READ STEP 3
Optics Type Luminescence Fiber
Filter Set <none>
Gain 80
Read Height 1.00 mm
Results Analysis
The Luminescence Test procedure is described on page 119 .
1. Determine if the plate’s battery is functioning properly. If A8 > (0.2 * A7), the
battery is good. Otherwise, it requires replacement.
A replacement battery is included with each new and recalibrated Harta
Luminometer Reference Microplate.
2. On the Harta plate’s calibration certificate, locate the NIST measurement for the A2
position. Convert it to attomoles : (A2 NIST measurement * 0.02884)
3. Calculate the signal-to-noise ratio : (A2–Mean of the buffer cells)/(3 * Standard
deviation of buffer cells)
4. Calculate the detection limit : A2 NIST measurement in attomoles/signal-to-noise
ratio
Synergy H1 Operator's Manual
Luminescence Testing | 85