Biotage VacMaster 10, User Manual

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VacMaster applies a vacuum to the outlet of a solid phase
extraction column (or similar) to assist in the extraction of
aqueous or viscous samples or to simultaneously process
multiple samples. VacMaster uses a vacuum controller with
fine and coarse adjustment controls and a vacuum gauge to
set and monitor the vacuum applied to the glass tank. The lid
seals to the tank with a gasket assisted by the vacuum.
Applying vacuum to the tank and opening the stopcock will
draw the sample or eluent through the SPE column or other
sample preparation product. The sample or eluent collects
in test tubes or vials held in the rack below the delivery tips.
Alternatively, using VacMaster without a rack, the solvent can
collect in the bottom of the tank. The glass tank has a vacuum
outlet fitting that can be oriented two ways. When the fitting
is turned up, solution will remain in the tank below the level
of the outlet fitting. When the fitting is swiveled down, waste
fluids in the tank flow into a collection trap for proper disposal.
Features
Easy to Access Stopcocks and Room
for Large Volume Columns
VacMaster manifolds are available with 10 or 20 positions.
With generous spacing between positions, you can use every
position every time, even with stopcocks and large volume
columns. VacMaster 10 can accommodate up to 10 x 70 mL
columns and VacMaster 20 up to 20 x 25 mL columns.
A Rack System That Is Easy to Adjust
to a Variety of Collection Tubes
The continuously adjustable rack system uses unique
threaded rods to secure the rack plates where you need them.
Rack plates are available for 10, 12, and 16 mm diameter
tubes as standard (plus 25 and 27 mm for VacMaster 10 and
20 mm for VacMaster 20). Replacement racks are available.
The maximum tube diameter possible with VacMaster 10 is
27 mm (accommodates scintillation vials) and 23 mm with
VacMaster 20. Custom racks are available, contact your
Biotage representative for details.
Needles with Easy to Use Stopcocks
That Do Not Contaminate Samples
The one piece needle design with a straight through flow path
will not trap samples or eluents, which could contaminate the
subsequent sample. The disposable needles consist of 316
stainless steel and will not corrode like stainless-steel-clad-
copper needles. PTFE retainers hold the stainless steel needles
in place, so they are easy to remove and replace. The inert
construction of the PTFE needle or combination needle stopcock
eliminates plasticizer contamination and ensures smooth
stopcock operation. Tapered PTFE tips eliminate overspray and
direct the sample and eluent into the collection tube. All of the
needles are securely threaded into the manifold top so they will
not pull out when removing the SPE column.
Materials of Construction That Will
Not Contaminate Samples
VacMaster is manufactured from high quality materials that will
not contaminate the samples being processed. The materials
include glass, PTFE, UHMW polyethylene, polypropylene, and
316 stainless steel. The thick walled glass tank provides a clear
view of the extraction process.
Vacuum Controllers That Will Not Become
Contaminated or Corroded
The precision made vacuum controller with gauge provides
excellent sample flow control. The vacuum regulator, placed on
the lid, is easy to see and control. Column eluents will not pass
by and contaminate or corrode the regulator.
Safe, Easy Way to Handle Biological
and Hazardous Sample Matrices
With the lid-mounted vacuum controller, it is possible to use
one tank to process the samples and dedicate a second low
cost tank for the elution step. Thus, the second tank can remain
uncontaminated. Additionally, the vacuum outlet fitting on the
glass tank can be oriented two ways. Turn the fitting up to close
the outlet and add the appropriate sterilization solution to the
tank to neutralize the collected biological solutions. Swivel the
fitting down to direct the waste fluids out of the tank into a side
arm collection flask for proper disposal.
Principle of Operation
Principle of Operation