Biotage VacMaster 10, User Manual

Page: 10 / 16

8 Biotage
®
VacMaster
™
10 & 20 User Manual | © Biotage 2020
Sample Preparation Columns (for
Example SPE, SLE, Filtration)
If you are uncertain as to which column to use, please contact
Biotage for technical advice or assistance.
Collection Tubes
Select collection tubes suitable for your rack and your intended
use. Racks are available to accommodate 10, 12, or 16 mm tubes
(and 25 or 27 mm for VacMaster 10 and 20 mm for VacMaster 20).
Installing the Needles
The stainless steel needle retainers are securely threaded into
the lid when VacMaster is assembled, and fitted with stainless
steel needles.
PTFE needles or combination needle stopcocks can be used in
place of the stainless steel needles. To install these needles,
remove the stainless steel needle retainers by rotating them
counterclockwise until they are free from the lid. Place the new
PTFE needle or combination needle stopcock into position and
turn clockwise until firmly seated against the lid. Use caution
to thread the needle correctly. Misthreading or overtightening
the needles or needle retainers could damage the threads on
the needles, retainers, or lid ports.
Installing Collection Tubes
The collection tube rack comes pre-assembled and should
be ready to use with most collection tubes. Simply place the
collection tube in the rack under each position you intend to use.
If your tubes do not fit in the rack or are not securely held by the
collection tube rack, you may need to replace the rack plates or
adjust the rack.
Replacing Collection Tube Racks
VacMaster is shipped with tube racks for 10, 12, or 16 mm tubes
(and 25 or 27 mm for VacMaster 10 and 20 mm for VacMaster 20),
specified with each order. Compare the part number of your
VacMaster with the ordering information on page 11 to
determine which rack you have. Alternatively, measure the
diameter of the opening in the top collection tube rack plate.
Additional 10, 12, or 16 mm collection tube racks or rack plates
are available. Custom racks that meet other collection tube
requirements can be purchased. See”Ordering Information”
on page 11 for part numbers and ordering information.
Adjusting the Collection Tube Rack
If your collection tubes are not securely held by the collection
rack, the rack may need adjustment. Each plate is held in
place by nut washers positioned above and below the plate.
When adjusting the rack, remember to keep all plates level.
Refer to Figure 4 on page 6 while adjusting the rack.
Bottom, Middle and Top Plates
Raise the Plate
Turn the nut washers above the plate counterclockwise until
they reach the correct height. Turn the nut washers below the
plate counterclockwise to raise the plate and tighten to
secure in position.
Lower the Plate
Turn the nut washers below the plate clockwise until they
reach the correct height. Turn the nut washers above the plate
clockwise to lower the plate and tighten to secure in position.
Handle and Dome Nuts
Securely tighten the dome nuts above the handle at the top
of the rack legs at all times, to provide a secure handle for
inserting or removing the rack from the tank.
Installation