Biotage Selekt Enkel, User Manual

Page: 8 / 44

6 Biotage
®
Selekt Enkel User Manual | © Biotage 2022
Prepare the System
Figure 14. The Solvent Setup window with solvent and waste monitoring
enabled.
Prime the Solvent Inlets
Before you start a purification on your system, you might need
to prime the solvent inlets to:
»
Remove any air bubbles from the pump and the
solvent inlets by flushing them with solvents.
»
Empty the solvent inlets of solvents used in the previous
purification and fill them with new solvents.
Note: Both solvent inlets must be primed with solvent to
achieve the specified pump performance.
Note: We recommend to have both solvent reservoirs on the
same physical height to improve accuracy in the solvent mixing.
1. In the software, press Menu and then Solvent Setup .
2. Prime the solvent inlets by pressing the corresponding
Prime buttons (see Figure 14). Note that 27 mL of the
assigned solvent is used for each prime.
Flush the System
If the automatic line flush is disabled on your system (see
page 24), ensure to flush the column flow path before each
run (see page 15).