Biotage Selekt Enkel, User Manual

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14 Biotage
®
Selekt Enkel User Manual | © Biotage 2022
Start, Monitor, and Control a Purification
Pause, End, or Abort a Purification
Note: Pausing a purification may cause gradient inconsistency
due to heat, solvent and sample diffusion, etc.
Pause and Resume a Purification Run
1. To pause the purification in progress, press .
The collection arm returns to its home position (in the
inner right corner) and the system is paused.
2. To resume the run from the point at which it was paused,
press .
End the Equilibration Step or
End or Abort the Purification Run
1. Press . The collection arm returns to its home position
(in the inner right corner) and the system is paused.
2. Press .
3. When the
System Paused
dialog opens, select one of the
available options:
»
Skip Equilibration: End the equilibration
step and move on to the gradient.
»
Abort: End the purification run without
performing any flushes.
»
Cleanup: End the purification run and perform the
flushes that are enabled in the system settings
and a system decompression (purge).
Edit a Purification
Edit on the Fly
The following run parameters can be changed without pausing
the purification run:
»
The run comment in the Information panel.
»
The flow rate in the Column panel.
»
Gradient composition, length and number of segments.
»
All collection parameters in the Collection panel
except for the UV baseline correction; see “Specify
the Collection Parameters” on page 9.
All changes except for changes to the gradient are instant.
When editing the gradient, the modified gradient is purple and
the ongoing gradient is black (see Figure 32). The modified/
purple gradient is applied when you press .
Figure 32. When editing the gradient while the system is processing,
the modified gradient is purple and the ongoing gradient is black.
The modified/purple gradient is applied when you press .
Pause to Edit a Purification in Progress
With the system paused, it is not only possible to change all the
run parameters that can be changed without pausing (see “Edit
on the Fly” above), but it is also possible to change the
Max
Fraction Volume setting in the Rack panel.
1. Press . The collection arm returns to its home position
(in the inner right corner) and the system is paused.
2. Edit the run settings; see “Set Up a Purification” on
page 7. To undo all changes, press .
3. To apply the changes and resume the run, press .
Confirm Cleanup
If the Confirm Cleanup option is enabled on the system, the
Gradient Run Completed dialog appears before cleanup is
performed, giving the user the option to manually extend the
run (see Figure 33). To extend, press
Extend Run , edit the run
settings, and then resume the run. By default the gradient is
extended with 25% of the total gradient length using the final
conditions of the run.
Figure 33. If the Confirm Cleanup option is enabled on the system,
the Gradient Run Completed dialog appears before cleanup is performed,
giving the user the possibility to extend the run. The dialog can be
minimized by pressing the arrows in the title bar.