Biotage Selekt Enkel, User Manual, Page: 11 / 44

9
Set Up a Purification
Specify the Information Parameters
»
User: The name of the user. If you have not been assigned
a user name, please contact your system supervisor.
»
Run Name: The name of the run. If left blank, the
name will be auto-generated based on date and
time when the purification is performed.
»
Comments: Comments on the purification run. (Optional.)
Figure 20. The Information panel expanded.
Specify the Column Parameters
»
Sample Mass:
The crude sample mass.
»
Type: The column type. Either scan the column using
the QR reader underneath the touch screen or select
a column type from the drop-down list. If a column is
scanned, the icon is shown in the
Column panel.
If the column name is orange or red, its load capacity
is smaller than the entered sample mass.
Note: If using a column or a combination of a column and
e.g. dry load vessel with a total CV larger than 0.8 L, a safety
valve supplied by Biotage (P/N 417115SP) must be used.
»
Flow Rate: The default (recommended) flow rate
for the selected column type is preselected.
If you want to change it, consider that the maximum
flow rate applied depends on the following:
»
The maximum pressure or flow rate setting for the
column and any other accessories used in the setup.
»
The maximum aspiration rate(s)
defined for the used solvent(s).
Note: If you expand the Column panel, all of the settings
for the selected column type are displayed (see Figure 21).
If the column has been scanned, the column’s ID and the
number of times it has been used is also displayed. For more
information, see “Administrate Column Types” on page 22.
Figure 21. The Column panel expanded.
Specify the Collection Parameters
»
λ -All
*
: Select whether the average absorbance within
a user-defined wavelength range should be used for
collection and fractionation (
Collect ), used for monitoring
( Show ), or not be shown during the run ( Hide ).
»
UV1 and UV2: Select whether the wavelength signals should
be used for collection and fractionation ( Collect ), used for
monitoring ( Show ), or not be shown during the run ( Hide ).
»
Wavelengths * : The shortest and longest wavelength to be
included in the
λ
-All signal. The range is 200 to 400 nm.
»
Wavelength: The wavelength to be used for
UV1 and UV2. The range is 200 to 400 nm.
»
Threshold: Used to collect samples when the
signal level exceeds the set threshold.
»
Collect All: Used to collect the entire run.
»
UV Baseline Correction
*
: When this option is turned on,
the gradient run is preceded by a light absorbance
detection phase. During this phase, the light absorbance
of the used solvents (A and B) is measured for the
whole detector range using either the initial gradient
mix to the end mix A/B or the initial gradient mix to
100% solvent B (see “System Settings” on page 24).
The measurement results in a baseline containing the
maximum absorbance of the solvents. During the gradient
run, the baseline is subtracted from the detector signal
affecting UV1, UV2,
λ
-All, and the absorbance spectrum.
* Only available on systems with a Spektra software license.