Biotage ELSD-1080, Operation Manual

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Biotage ELSD-1080
Method
Number
Evaporator Temp
(°C)
Nebulizer Temp
(°C)
Gas Flow
(SLM)
1 30 30 1.6
2 25 30 1.6
3 20 30 1.8
4 15 30 2.2
5 20 80 1.2
6 40 40 1.4
7 50 50 1.0
8 80 60 1.0
9 80 80 0.9
10 80 90 3.0
Table 4. Ten Control Methods Pre-loaded on Biotage ELSD-1080
3.4.2 Evaporator Temperature
The evaporator temperature is the most important setting on
the ELSD-1080 detector. This should be set according to the
volatility of the compound(s) being analyzed.
If the compound is non-volatile, e.g. sugars, then the
evaporator temperature should be set to 80-90 °C.
If the compound is semi-volatile, or has a low molecular
weight, e.g. pharmaceutical drug, then the evaporator
temperature should be set between 20-30 °C.
The evaporator temperature range for the ELSD-1080 is 10-80 °C
(1 °C increments). The default evaporator temperature is 30 °C.
3.4.3 Nebulizer Temperature
The nebulizer temperature can be used to optimize signal
response in addition to evaporator temperature. Higher
nebulizer temperatures increase peak response, but the
nebulizer temperature must not exceed the boiling point of the
mobile phase.
The nebulizer temperature range for both models is: OFF, 25-
90 °C (1 °C increments). The default value is 30 °C.
3.4.4 Evaporator Gas Flow
The evaporator gas flow is used to control the ELSD-1080
evaporation process. The evaporator gas value is set according
to the mobile phase composition, with higher gas flows (e.g.
1.6 SLM) being used for aqueous eluent compared to those
containing organic solvents.
The higher the evaporator temperature, the lower the
evaporation gas setting required (e.g. 1.0-0.9 SLM), regardless
of mobile phase composition. Likewise, as the evaporator
Publication date: July 2010 35