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10.7. Troubleshooting
Table 5. Troubleshooting
Symptom
Possible cause
Action required
Manifold dosing error
Volume of washing
solution does not
correspond to the set
volume, dosing
unevenness is
observed along the
plate wells or the
washing solution is
not dispensed at all.
Poor contact between the
bottle hose connector and
the device
Ensure proper connection of the bottle with the
device.
The hose is overbent
Check if the hose is overbent and straighten it if
necessary.
Obstruction of sieve filters
Check if the sieve filters in bottles No. 1, 2 and 3
are clogged.
Obstruction of dispensing
channel of the manifold or
a needle
Check if the dispensing channel of the manifold or
needles is not obstructed. If yes, perform cleaning
(see “Obstruction of dispensing channel or a
needle i
n the manifold” in this Table).
Absence of liquid in bottles
Ensure presence of liquid in the bottles No. 1, 2
and 3; fill up liquid in a bottle if needed. (Attention:
with 4CHW Logger, information on liquid levels in
bottles is shown on the display).
Obstruction of
dispensing channel
or a needle in the
manifold
During operation needles
of the manifold can be
obstructed.
Determine the obstructed needle by checking the
underfilling of a well.
Warning!
Be careful working with the manifold,
prevent pricks and injuries caused by needles, they
may contain dangerous infections!
Take off the manifold from the device, put it down
with needles upward. Clean the aspiration channel
needles with the wiping rod Ø 0.80 mm, and clean
needles of the dispensing channel with the wiping
rod Ø 0.45 mm, inserting the wiping rod against
stop and removing it.
After cleaning, install the manifold on the device
and do the rinsing procedure to ensure proper
operation of the needle.
In case of repeated obstruction, execute
requirements of pp. 1 and 2, unscrew the
manifold’s plugs, disconnect the dispensing and
aspirating tubes, clean the dispensing or aspiration
channel with the wiping rod, rinse the manifold with
the 75% solution of ethanol, replace the plugs,
install the manifold on the device and ensure the
proper operation of needles.
Washing solution is
not dispensed into a
plate
Deformation (wall gluing)
of dosing valve tube due to
the long-term storage or
standstill of the device
Remove the tube from the valve.
In the case of deformation, open with fingers the
closed part of the dosing valve.
Insert the tube into the valve opening to position
shown in fig. 2/4.
Note:
In order to release the tube, press the valve
on the side and slightly stretch the tube pulling it
through the opening.
Wear or damage of
the valve tubes and
the pump
The connecting tubes are
worn out or damaged
during exploitation.
Turn off the device and have it checked by a
specialist. Only service engineers and trained
specialists are allowed to replace the tubes.
