12
5.3.
All possible program parameters and their meaning are shown in Table 2.
Table 2. Program parameters and their definitions
Parameter
Value or range
Description
Parameter
Value or range
Description
Dispense
yes/no
Perform fill
Aspirate
yes/no
Perform aspiration
1
Shake
yes/no
Plate shaking on the platform during the cycle
Dispense rate
01..03
Speed of liquid filling (100; 200; 300 μl/s)
Aspirate rate
01..03
Speed of liquid aspiration
Soak limit, s
0..300 (step 10 s)
Time between fill and aspiration
Shake limit, s
05..150 (step 5 s)
Shaking time
Double aspiration
yes/no
Second aspiration mode
Dispensed volume
25…1600 (step 25)
Volume of dispensed liquid
Aspirate time, ms
200..3000 ms (step
200 ms)
Time of aspiration from well in cycle
Final aspirate, s
200..3000 ms (step
200 ms)
Time of last aspiration in the cycle
First aspirate
yes/no
Aspiration is the first action in a cycle
Wash by rows
yes/no
Each row is washed once per cycle
On two channels
yes/no
Use 2 washing solutions
Num.of wash cycles
1..15
Number of washes with first solution
1 channel
1..3
Number of bottle to use for the main washing
Second chan. cycles
01..15
Number of washes with second solution
2 channel
1..3
Number of bottle to use for the second washing
5.4.
Install the plate on the platform.
Attention!
When installing a plate the first time or when a different type of plate
is installed perform plate setup to adjust the manifold lowering depth
(see paragraph 4.20).
5.5.
Use the
+
and
-
keys (fig. 4/6) to select required washing program from 00 to 100.
5.6.
Press the
Program Parameters (Save)
key (fig. 4/1) to view the parameters of
program. In the program, 1st parameter is activated (Dispense...yes/no).
5.7.
Use the
+
and
-
keys to select necessary value and press the
Enter/Calibr.
key (fig.
4/4) to save changes to the active parameter and proceed to the next. This way you
will be able to review and/or change all parameters.
5.8.
Press the
Back/Row
◄
key (fig. 4/3) to return to the previous parameter in the
program.
5.9.
Press the
Program Parameters (Save)
key to exit parameter view and change mode
and save the program.
5.10. Press the
Esc
key (fig. 4/5) to exit parameter view and change mode without saving.
5.11. Press the
Run/Stop
key (fig. 4/7) to start the program.
1
During aspiration, the waste fluid bottle is detected automatically
– bottle #4. The number is not
specified on the display
