BiOptic Qsep1 Operation Manual Download Page 7

 

1.

 

System Overview

 

Qsep

™ 

Series are a fully-automated CE system developed by BiOptic Inc., which uses 

pen-shaped disposable gel-cartridges to improve efficiency. Time-consuming manual 

procedures such as gel preparation, sample loading, and capillary changing are no 

longer required. Further, the information will be obtained easily with the fully- 
automated 

Qsep

™ 

Series.

 

Qsep

100

™ 

and

 

Qsep

1

 

are designed to accommodate the 

standard 96-well and 8-12 well PCR plate, respectively. 

 

Qsep

™ 

Series

’s

 compact design helps you to set up and operate the instrument 

intuitively. The disposable gel-cartridge with integrated pre-programmed test 

methods makes capillary electrophoresis experiments no longer a painful procedure 

that requires well-trained operators. No more worries about operation and human 

errors caused by different operators which affect the accuracy and reproducibility of 

the results. 

 

The following sections will describe the product overview, the functions of

 

Qsep

™ 

Series instruments and the gel-cartridge. Please read through this section and get 
ready to be amazed with the power of 

Qsep

™ 

Series.

 

 

Caution: 

The operator of this instrument is advised that if the equipment is 

operated in a manner not specified in this manual, the protection provided by the 

equipment may be impaired. 

 

 

Summary of Contents for Qsep1

Page 1: ...BiOptic Inc 4F No 108 3 Minquan Rd Xindian Dist New Taipei City 23141 Taiwan R O C www bioptic com tw Revolution Qsep1 Qsep100 Operation Manual Hardware...

Page 2: ...is prohibited without prior written permission of BiOptic Inc except as allowed under the copyright laws Qsep Series and Q Analyzer are registered trademarks of BiOptic Inc Microsoft Windows is regist...

Page 3: ...0 1 1 3 Qsep100 Top View 11 1 2 Qsep1 Instrument 12 1 2 1 Qsep1 Front View 12 1 2 2 Qsep1 Back View 13 1 2 3 Qsep1 Top View 14 1 3 Hardware Requirements 15 2 Installation Instruction 16 2 1 Environmen...

Page 4: ...r loss resulting from not complying or familiar with the manuals or improper operation of the devices Applications Genetic Profile Screening Amplified Fragment Length Polymorphisms Bacterial Viral Gen...

Page 5: ...ce it upside down 4 Store the cartridge in the calm shell container Cartridge needs to be stored in a proper environment as the instructions of cartridge unpacking guide to maintain its lifetime and q...

Page 6: ...ease disconnect the power plug 15 Please clean the instrument with dry cloth For other maintenance please contact local distributor 16 Please use the MAINS power cord within adequate rating 17 The con...

Page 7: ...ument intuitively The disposable gel cartridge with integrated pre programmed test methods makes capillary electrophoresis experiments no longer a painful procedure that requires well trained operator...

Page 8: ...when the high voltage source of the capillary electrophoresis is ON B Main Power switch The blue LED lights up when it is turned on C Operation room Open the sample door A rotation platform includes...

Page 9: ...9 Figure 1 2 Rotation platform...

Page 10: ...p100 back view A Air pressure gauge Display the value of the pressure operating in Qsep100 B Air flow Inlet Port Connect to an external pump using an 1 8 inches tube C USB Port Connect to a computer D...

Page 11: ...11 1 1 3 Qsep100 Top View Figure 1 4 Qsep100 top view A Cartridge door...

Page 12: ...12 1 2 Qsep1 Instrument 1 2 1 Qsep1 Front View A Indicator lamp B SD Card Port C Cartridge Door D Sample Door and Sample Tray...

Page 13: ...13 1 2 2 Qsep1 Back View A Power Connector B Main Power switch C Air flow Inlet Port Connect to an external pump using an 1 8 inch tube D Cable Port...

Page 14: ...14 1 2 3 Qsep1 Top View A Cartridge door A...

Page 15: ...are Requirements Minimum Recommended CPU Intel Core i3 530 2933MHz Dual Core Intel Core i7 2 8 GHz RAM 4G RAM or More 8G RAM or More HDD 320GB or More 500GB or More PORT USB 2 0 x 1 USB 3 0 x 1 INTERN...

Page 16: ...installation it is recommended to leave approximately 711 mm 28 inches clear space above the counter space or table Allow approximately 76 mm 3 inches of clear space at the rear and to the side of the...

Page 17: ...source within the range of 50psi 75psi The input voltage of Qsep1 instrument is 12V DC and the maximum power is 80W A suitable power adapter with cord MEAN WELL GSM90B12 P1M is included in the package...

Page 18: ...inside the portable air pump If this occurs be sure to release the condensation water before using Qsep Series If you need to continuously operate Qsep Series check the air pump every 12 hours to avoi...

Page 19: ...r WiFi function to connect the instrument with your computer after Turn on Qsep1 Turn on the power wait 25 second for WiFi function initialization Green light flashing and install the operation softwa...

Page 20: ...t the air tube 3 Plug the power cord labeled Qsep1 4 Switch on Qsep1 the LED lights multiple color light Fig 3 Wait 25 seconds for the instrument and Wi Fi initialization and the green LED will start...

Page 21: ...sword The password will show in the sticker which is behind the instrument It is the same as the middle 8 digits of instrument serial number same as SSID name by blinding the prefixal Qsep1 Fig 7 If y...

Page 22: ...the firewall 5 Check the LED status 6 Double click the Q Analyzer 7 Q Analyzer user interface 7 1 Select Network mode and click Search After Search the instrument ID will display Please confirm the in...

Page 23: ...es Make sure no air bubble appears in each tube 9 Click Change Sample or Change Buffer the sample door will open automatically 10 Slide the buffer tray into the instrument Make sure sample tray is pus...

Page 24: ...n New cartridge needs to be calibrated before using Please proceed with the following steps 14 1 Click HV Check The storage and transportation condition may influence the Gel matrix and cause unstable...

Page 25: ...will only display after HV check passed For troubleshooting please refer to cartridge unpacking guide for details 15 To analyze samples click on the blank column and designate the sample locations tes...

Page 26: ...lect a proper method for your application 15 3 Click on Result Name and enter the name of result files 15 4 Click the icon Para and set the parameters Baseline Factor Peak Threshold Calculate etc 16 C...

Page 27: ...ease check the Alignment Marker has been located at correct position Software will recognize two peaks from Alignment Marker signal DO NOT use Size Marker or DNA sample doing Calibrate Brief introduct...

Page 28: ...28 3 2 Qsep100 Quick Start 1 Turn on the power 2 Double click the Q Analyzer 3 Main window appears 4 Click Connect to link the instrument 5 Cartridge and Buffer preparation...

Page 29: ...into the 0 2 ml tube and then add 10 l Mineral Oil on the top of Alignment Marker 6 Click Change Buffer 7 Place Alignment Marker 20 bp 1000 bp C109100 20 l at MA 1 position and the buffer tray on the...

Page 30: ...the sample is in the bottom of tube and no air bubble appears 10 According to cartridge unpacking guide open the cartridge door and insert cartridge Guiding groove facing front 11 Close the cartridge...

Page 31: ...ransportation condition may influence the gel matrix and cause unstable current Check current and see if it s stable or not After HV Check if current is still unstable please repeat this step 2 3 time...

Page 32: ...plate and then press OK 13 2 Click Method to select analysis method If you want to analyze the samples with Alignment Marker please choose the appropriate Alignment Marker and then place it on the cor...

Page 33: ...etc 14 Click Run to start the process During Calibration Please check the Alignment Marker has been located in correct position Software will recognize two peaks from Alignment Marker signal DO NOT u...

Page 34: ...34 Introduction of the Control Panel...

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