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CONTENTS

Page/s

1. Biohit 

e

LINE electronic pipettors

2

1.1. Biohit 

e

LINE single-, 8- and 12-channel pipettors

2

1.2. Biohit 

tips

3

2. Unpacking

3

3. Pipettor 

description

4

3.1.  Display and controls

4

3.2. Reset 

button

6

3.3. Start 

button

6

3.4.  Tip sealing and Electronic tip ejection 

6

3.5.  Biohit Safe-Cone filters

7

4.  Charging of the pipettor

8

4.1.  Charging with the 

e

LINE Charging Stand and Carousel

8

4.2.  Charging through the direct charging system

9

4.3. Electrical 

specifications

10

5. Programming 

principle

10

5.1. Programming 

map

11

5.2. Speed 

adjustment

12

6. PIPET 

modes

12

6.1. Pipetting 

mode 

) and pipetting with mixing ( P+

MIXING

)  

12

6.2.  Reverse pipetting ( rP )

14

6.3.  Pipetting with cycle counter ( 

COUNT

)

15

6.4.  Manual pipetting ( P

MANUAL

)

16

7. MULTI 

modes

17

7.1. Multi-dispensing 

)

17

7.2. Diluting 

dd ) and diluting with mixing ( dd+

MIXING

)

19

7.3. Sequenced 

dispensing 

Sd )

20

7.4. Multi-aspirating 

SA )

21

7.5.  Automatic dispensing ( Ad )

22

8. PROG 

modes

24

8.1.

Store operating mode to storage location

24

8.2.

Recall stored mode from storage location

24

9. Pipetting 

recommendations

25

9.1.  Dispensing with blow-out

25

9.2.  Dispensing without blow-out

25

9.3. Other 

recommendations

25

10. Maintenance

26

10.1.  Cleaning the outer surface of the pipettor

26

10.2. Cleaning and autoclaving the lower part of the 1-ch pipettors

26

10.3. Battery replacement

28

10.4. Storage

29

11.

Performance Testing 

29

11.1. Recalibration

31

12. Trouble-shooting

32

13. Warranty information

34

14. Performance specifications

34

1

Summary of Contents for eLINE 73002X

Page 1: ...e pipetting rP 14 6 3 Pipetting with cycle counter P COUNT 15 6 4 Manual pipetting P MANUAL 16 7 MULTI modes 17 7 1 Multi dispensing d 17 7 2 Diluting dd and diluting with mixing dd MIXING 19 7 3 Sequ...

Page 2: ...e keyboard and easy to read display together with user friendly operation and programming allows these tasks to be performed quickly and effortlessly The Biohit eLINE pipettors operate on the air disp...

Page 3: ...rage with a plastic protector BEFORE USE PLEASE REMOVE THE PLASTIC PROTECTOR UNDER THE BATTERY COVER Note It is recommended to recharge the pipettor for at least two hours before initial use The pipet...

Page 4: ...ousing Tip Ejector Bar Tip Cone s excl 5000 Safe Cone Filter Battery Cover Charging Contacts Reset Button Finger Hook Tip Cone e5000 Tip Ejector Assembly e5000 3 1 Display and controls The control and...

Page 5: ...ction buttons Reset button Triggers all piston Press to remove tip Press to reset the movements pipettor Mode Ranges and Operating Mode Symbols PROG PROG 1 6 6 storage locations MULTI d multi dispensi...

Page 6: ...piston will stop in the lowest position until the button is released In the operating modes with automatic blow out P dd the piston will automatically return to the home position after the start butt...

Page 7: ...not have an adjustable tip ejector collar 3 5 Biohit Safe Cone Filters The tip cone design of the Biohit eLINE pipettors 10 l allows the use of Biohit Safe Cone Filters in the tip cones These removabl...

Page 8: ...t eLINE Charging Stands and Carousels are bench top charging units The Charging Stand accommodates one Biohit eLINE pipettor and the Charging Carousel four Biohit eLINE pipettors The eLINE Charging St...

Page 9: ...it is recommended to keep the pipettor in the Charging unit This ensures that the battery is kept charged and the pipettor is ready for use 4 2 Charging through direct charging system The AC adaptor s...

Page 10: ...mode range is selected with the Select key the desired operating mode is then selected with the arrow key and selection confirmed with Enter key The setting changes volumes aliquots etc are made with...

Page 11: ...change settings When pre stored including speed excluding speed Prog 1 6 was chosen Start pipetting Store the operating mode with Change selected settings with settings to Prog 1 6 Start pipetting P M...

Page 12: ...udes 5 different operating modes 6 1 Pipetting mode P and pipetting with mixing P MIXING In pipetting mode P the eLINE performs pipetting with blow out In pipetting with mixing mode P MIXING pipetting...

Page 13: ...ut and the piston returns automatically back to the home position Step 10 Step 11 Note When the START button is held down during dispensing the piston will stop in the lowest position When the start b...

Page 14: ...7 Press or to confirm selection and to display the speed out 8 Select the speed out with 1 Min and 9 Max 9 Press or to confirm selections 10 Position the tip to aspirate and press the START button 11...

Page 15: ...irm selection 6 Cycle counter will show 1 Use to select the sample number you want to begin with 0 99 7 Press to confirm the selection and to display the speed in or press and continue from Step 12 8...

Page 16: ...is displayed 3 Confirm by pressing 4 Select the desired pipetting volume by using Note When holding down the volume display starts to scroll faster 5 Press to confirm selection and to display the spe...

Page 17: ...dispensing of a selected volume The sum of the dispensing aliquots and an automatically selected excess volume is aspirated into the tip The excess volume is needed to ensure equal operating conditio...

Page 18: ...own to indicate the reset function 13 Position the tip to discard priming excess and press the start button 14 To dispense position the tip press START button and repeat until the cycle is complete 15...

Page 19: ...ing Note When holding down the volume display starts to scroll faster 5 Press or to confirm the selection 6 Select the desired sample volume volume 2 with 7 Press to confirm selection and to display t...

Page 20: ...volumes of same solution can be delivered in any desired order The sum of dispensed volumes can not exceed the nominal volume of the pipettor 1 Press to display MULTI 2 Scroll with until the symbol Sd...

Page 21: ...button twice Note It is also possible to continue to aspirate and dispense the same volumes without the empty function To continue keep the START button pressed during the last dispensing Step 16 and...

Page 22: ...ete The total volume is displayed 13 Position the tips to dispense and press START button twice Step 12 Step 13 7 5 Automatic dispensing Ad The pipettor performs repetitive dispensing of a selected vo...

Page 23: ...E OUT and arrow down to indicate the reset function 15 Position the tip to discard priming excess and press the START button 16 To dispense position the tip press the START button once and the pipetto...

Page 24: ...g 1 6 locations 1 Complete the programming of the selected operating mode as described in paragraphs 6 and 7 2 Press first and then simultaneously to display the PROG 1 3 Choose the desired storage lo...

Page 25: ...west position This allows the tip to be placed under the liquid surface or against the bottom or the wall of the container during dispensing Once the liquid is dispensed the tip can be removed from th...

Page 26: ...ed always to use gloves when cleaning the pipettor Change the tip cone filter regularly with the tool provided 10 1 Cleaning the outer surface of the pipettor To clean and decontaminate the outer surf...

Page 27: ...jector collar 2 tip cone holder 3 tip cone 4 spring 5 and piston 6 into a beaker containing Biohit Proline Biocontrol and leave for at least 30 minutes Fig 9 rinse the parts with distilled water Note...

Page 28: ...pettor after in house service or maintenance see paragraph 11 10 3 Battery replacement The design of the Biohit eLINE pipettors enables fast and easy battery replacement The battery replacement pack i...

Page 29: ...est water should have stood in the test room sufficient time at least 2 hours to reach equilibrium with the room conditions Use distilled or de ionised water grade 3 Use an analytical balance with a r...

Page 30: ...ce between the dispensed volume and the selected test volume Random error is the scatter of the dispensed volumes around the mean of the dispensed volume ISO 8655 1 Note Biohit specifications are achi...

Page 31: ...selected volume at a time as follows 1 Press to display PIPET 2 Scroll with until the desired mode P rP P MIXING or P COUNT is displayed 3 Confirm the selection by pressing 4 Select the pipetting volu...

Page 32: ...action If any error message appears to the display it means that the pipettor has been unable to perform the attempted action properly In case of an error the display will show Error and PRESS Ent mes...

Page 33: ...iston and the tip cone Insufficient amount of Grease accordingly grease on a piston and o ring Pipettor out of Instrument damaged Return to your established specifications Biohit service representativ...

Page 34: ...nic pipettor is tested before shipping by the manufacturer The Biohit Quality Assurance Procedure guarantees that the Biohit eLINE electronic pipettor you have purchased is ready for use Each Biohit e...

Page 35: ...8 ch 10 300 l 300 l 0 40 0 20 150 l 0 70 0 30 30 l 2 00 1 00 1 30 730390 8 ch 50 1200 l 1200 l 0 50 0 20 600 l 1 00 0 30 120 l 2 50 1 00 1 24 730420 12 ch 0 2 10 l 10 l 0 90 0 50 5 l 1 50 0 80 1 l 4...

Page 36: ...36 Notes...

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