Biognosys PlasmaDive Mini, Manual

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Second Edition, Version 2.03 April 2017
PlasmaDive
™ Mini Manual, © Biognosys AG, Switzerland
Page 5 of 22
Introduction: PlasmaDive
™ Mini Assay Panel at a Glance
Blood is the most frequently used biological sample in clinical research and routine
laboratory diagnostics. Levels of blood proteins reflect the health status of single
organs and the body as a whole. Changes in composition of proteins in the blood can
be correlated to disease onset or therapy response. Often the relative changes in
protein concentrations to each other are the key indicator of a certain condition. Only
monitoring the levels of major blood proteins simultaneously makes possible to
recognize the “Big Picture” and explain pathological processes going on in the body.
The PlasmaDive
™ Mini Assay Panel was designed for targeted proteomics
approaches - Multiple Reaction Monitoring (MRM, also called SRM) or Parallel
Reaction Monitoring (PRM) - which focus on quantifying predefined sets of proteins
with high sensitivity and reproducibility. Biognosys is unique in its ability to perform
scheduled highly multiplexed MRM and PRM measurements based on its innovative
iRT concept and its specifically developed signal processing software
– SpectroDive™.
Both, iRT and
SpectroDive™, are integral parts of Biognosys’ Assay Panels.
The PlasmaDive
™ Mini Assay Panel optimally combines 100 peptide MRM or PRM
assays in one scheduled multiplexed method. Each peptide is representative of a
human plasma protein (
Table 1 ). The PlasmaDive
™ Mini workflow requires as little as
10 µl of plasma and 48 hours to provide actionable clinically-relevant data.
Table 1. List of proteins quantified with PlasmaDive
™ Mini Assay Panel
UniPot ID Entry Name Protein Name
P02763 A1AG1_HUMAN Alpha-1-acid glycoprotein 1 (Orosomucoid-1)
P19652 A1AG2_HUMAN Alpha-1-acid glycoprotein 2 (Orosomucoid-2)
P01009 A1AT_HUMAN Alpha-1-antitrypsin
P04217 A1BG_HUMAN Alpha-1B-glycoprotein
P08697 A2AP_HUMAN Alpha-2-antiplasmin (Serpin F2)
P02750 A2GL_HUMAN Leucine-rich alpha-2-glycoprotein (LRG)
P01023 A2MG_HUMAN Alpha-2-macroglobulin (Alpha-2-M)
P01011 AACT_HUMAN Alpha-1-antichymotrypsin (ACT)
P43652 AFAM_HUMAN Afamin (Alpha-albumin)
P02768 ALBU_HUMAN Serum albumin
P35858 ALS_HUMAN
Insulin-like growth factor-binding protein complex acid
labile subunit
P02760 AMBP_HUMAN Protein AMBP
P01019 ANGT_HUMAN Angiotensinogen (Serpin A8)
P01008 ANT3_HUMAN Antithrombin-III (Serpin C1)
P02647 APOA1_HUMAN Apolipoprotein A-I
P02652 APOA2_HUMAN Apolipoprotein A-II
P06727 APOA4_HUMAN Apolipoprotein A-IV
P04114 APOB_HUMAN Apolipoprotein B-100
P02654 APOC1_HUMAN Apolipoprotein C-I
P02655 APOC2_HUMAN Apolipoprotein C-II
P02656 APOC3_HUMAN Apolipoprotein C-III
P05090 APOD_HUMAN Apolipoprotein D
P02649 APOE_HUMAN Apolipoprotein E
P02749 APOH_HUMAN Apolipoprotein H
O14791 APOL1_HUMAN Apolipoprotein L1