14
Short Instructions
• Plug the Decloaking Chamber into a 110-120 V power source with surge protection.
• Place the gasket in the lip of the Decloaking Chamber lid.
• Place the Decloaking Chamber pan into the Decloaking Chamber shell (body).
• Place the heat shield in the center of the pan.
• Pour 500 ml of deionized (DI) water into the pan.
• Ensure that the heat shield is in the center of the pan after the addition of DI water.
• Place slides into Tissue Tek racks.
• Place the Tissue Tek racks into Tissue Tek containers containing 250 ml of desired retrieval buffer (Example: Diva). Arrange
Tissue Tek Containers on top of the heat shield off-center to avoid heat concentration. Containers must not be covered.
• Note:
Do not use glass containers.
• Place a Steam Monitor Strip across the edge of the Tissue Tek containers.
• Align the shell and pan handles.
• Match the lid etching “OPEN” with the white dot on the pan handle.
• Hold both the shell and pan handle with your left hand while turning the lid clockwise with the right hand until the lid etching
that reads “CLOSED” is aligned with the white dot of the pan handle on the left side.
• The metal tabs on the lid should be tightly seated against the pan’s lip.
• Turn the instrument on by flipping the red toggle to the right of the control panel to the “ON” position.
• Press “DISPLAY SET” once, until the SP1 LED illuminates.
• The user can scroll through the instrument settings by pushing the “DISPLAY SET” button multiple times.
• Recommended factory settings for IHC are SP1 of 125 °C for 30 seconds, SP2 of 90 °C for 10 seconds, SP Safety Limit of
10 °C, unless otherwise specified by the data sheet.
• Push “Start/Stop” button to initiate programmed run.
• When the alarm sounds (approximately 10-15 minutes) press the “Start/Stop" button. The SP2 will illuminate. Record the
temperature and pressure.
• When the alarm sounds a second time (approximately 20 minutes) the temperature has cooled to your programmed SP2 temperature.
• Visually confirm that the pressure has dropped to 0 (zero) psi on the pressure gauge.
• Toggle the weight (petcock) to release any residual pressure. Turn the unit off.
• Open the lid with the steam directed away from yourself.
• Confirm that the Steam Monitor Strip has changed to a dark brown to black color (Figure 6b).
• Leave the containers in the Decloaking Chamber to cool down for 10 minutes.
• Carefully remove the Tissue Tek containers from the instrument.
• Decant half of hot retrieval solution and add DI water.
• Rinse with five changes of DI water.
• Place slides in buffer solution for five minutes.
• Proceed with immunohistochemistry (IHC) staining.
