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10

Programming Display

Control Panel

(See the inside back cover for a recommended settings guide)

Heat On light: 
Indicates that the heating element is heating up. 

Set light: 
Indicates that the current parameter, SP1, SP2 or SP Limit can 
be programmed. 

Actual light: 
Shows the actual immediate internal temperature. 

LED screen: 
Displays the current setting or error message.

Display Set button: Cycles the user through the two programs SP1 and SP2 (SP = Set Point). Pressing "Display Set" multiple times 
will navigate the user from SP1 temperature and time, to SP2 temperature and time, then to SP Limit, Actual Temperature and 
back to SP1. 

Up and Down arrows: Adjusts the settings for temperature and time higher and lower. 

Start/Stop button: Starts and stops the instrument depending upon the temperature and time settings. 

SP1 LED readout: Set Point 1 has two LED readouts on the digital display. The first readout is the temperature at which the user 
wants to set the Decloaking Chamber, the second is the time interval the user wants to maintain the SP1 temperature.

SP2 LED readout: Set Point 2 has two LED readouts on the digital display. The first readout is the temperature to which the 
chamber has to be cooled in order to be able to remove the slides. The second readout is the amount of time the SP2 temperature 
is to be maintained before an alarm goes off. The purpose of the second alarm is a quality control that allows for standardization 
between runs. It also ensures that the slides remain inside the chamber for a standardized amount of time.

SP Limit readout: The Set Point Limit is an internal circuit breaker that is set by temperature. It is an absolute limit on temperature 
that cannot be exceeded or the heating element will turn itself off. For example: if SP1 is set to 125 °C and the SP Limit if set to 
10 °C, the element will automatically turn off if a temperature of 135 °C is reached. This is a safety feature that avoids overheating 
if there is not enough water added to the system.

Error Messages

The LED panel will display the letters “ERR” if the following technical problems occur:

•   The pan is not filled with enough water, causing the heat and pressure to rise beyond normal limits

•   The SP Limit is set so low that the normal temperature tolerance heats beyond the SP1 + SP Limit

•   Failure of the Heat Sensor

Audible Alerts

When the chamber reaches the SP1 time or the SP2 time, the alarm will beep until the "Start/Stop" button is pressed. The temperature 
and pressure should be recorded at these set points for quality control.

Display Buttons: The display buttons beep each time they are pressed. 

DISPLAY

SET

START

STOP

Summary of Contents for Decloaking Chamber Pro

Page 1: ...DISPLAY SET START STOP C H A M B E R www biocare net U S patent number 6 580 056 Decloaking Chamber Pro Manual Digital programmable pressure system for heat induced epitope retrieval ...

Page 2: ...Biocare Medical Technical Support 800 799 9499 Option 3 Customer Service 800 799 9499 Fax 925 603 8080 Corporate Office 60 Berry Drive Pacheco CA 94553 www biocare net ...

Page 3: ...ncidental or consequential damages related to this equipment or for any warranty related services it performs Not for use with flammable solvents or gases Specifications 1 Assembly 2 Unpacking 2 Description of Parts 3 Related Parts Products 4 Operating Instructions 5 9 Setting Up 5 Recommended Protocols 5 Beginning Operation 5 Programming Display 10 Control Panel 10 Error Messages 10 Audible Alert...

Page 4: ... is of the utmost importance for consistent immunohistochemistry IHC staining The Decloaking Chamber is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety requirements Temperature pressure and pH can be monitored and recorded with the Decloaking Chamber to produce consistent staining which will reduce repeat testing and false ...

Page 5: ...e returning the instrument becomes necessary You should have Lid with pressure gauge and hot sticker Gasket removable Weight petcock Pan removable Body with three prong electric cord programmable digital timer Heat shield The Decloaking Chamber is compatible with 110 120 V A step down transformer is required for laboratories using a 220 240 V electrical source When necessary the step down transfor...

Page 6: ...in contact with the heat sensor and heat plate Vent Nozzle The top center of the chamber lid that where the weight petcock is placed Heat Shield A circular concave metal disc with perforations on it that is placed in the center of the pan Heat Sensor A circular spring loaded device within the main body that records the Actual Temperature during operation Heat Plate A large metal disc area within t...

Page 7: ... a lighter strip The steam strip can be dated and recorded for laboratory and CAP inspections Cat No 613 C D H QC Heat Tested pH Strips The pH of retrieval solutions at high temperatures can be critical for proper IHC staining However the pH of retrieval solutions can change at high temperatures To assure quality control and consistent staining Biocare Medical has developed heat tested strips that...

Page 8: ...sheet recommendations and protocols are based on exclusive use of Biocare products Ultimately it is the responsibility of the investigator to determine optimal conditions These products are tools that can be used for interpretation of mor phological findings in conjunction with other diagnostic tests and pertinent clinical data by a qualified pathologist Recommended protocols for HIER with Decloak...

Page 9: ...6 Operating Instructions continued 1 2 3 Add 500 ml of DI water Place the heat shield in the center of the pan at the bottom Place the gasket into the groove on the top of the pan ...

Page 10: ...al solution container Unused Steam Monitor Strip Steam Monitor Strip at 110 C or above for 30 seconds Place the container with the retrieval solution on top of the heat shield Do not place the container on the center of the heat shield where the heat is concentrated but towards the edge of the heat shield Do not use glass containers ...

Page 11: ... 8 9 Place the weight petcock onto the vent nozzle Make sure there is a snug flat connection Match the word OPEN on the lid to the white dot on the handle Secure lid by turning clockwise until the word CLOSED is matched with the white dot on the handle ...

Page 12: ...9 Operating Instructions continued Gap No Gap Correctly secured lid Gap indicates that the lid is not correctly secured ...

Page 13: ...o be able to remove the slides The second readout is the amount of time the SP2 temperature is to be maintained before an alarm goes off The purpose of the second alarm is a quality control that allows for standardization between runs It also ensures that the slides remain inside the chamber for a standardized amount of time SP Limit readout The Set Point Limit is an internal circuit breaker that ...

Page 14: ...to start the program Push the down arrow to display the actual temperature Both the Heat On and the Actual Temperature lights will be on When the programmed temperature is attained the Heat On light will turn off and the timer will start counting down The instrument will typically overshoot the programmed temperature by about 2 C to 4 C then settle down to the programmed temperature When the timer...

Page 15: ...play Set button again and use the up and down arrows to set the SP Limit Note To speed up the process of setting the time push the other arrow while holding the original direction arrow down Time Bypass Feature If the SP1 time is set to 99 00 minutes the Decloaking Chamber will maintain a continuous temperature without a time countdown Temperature cannot be set higher than 100 C as a safety precau...

Page 16: ...t the gasket for tears or wear If the vent nozzle becomes blocked with debris clear the path with a toothpick or similar device then wash clean Heat Plate and Heat Sensor Wipe off anything stuck to the heat plate or heat sensor and dry with a soft cloth Body Wipe the surface with a soft cloth soaked in warm water making sure that no water gets into the body Electrical Precautions Basic safety prec...

Page 17: ...s lip Turn the instrument on by flipping the red toggle to the right of the control panel to the ON position Press DISPLAY SET once until the SP1 LED illuminates The user can scroll through the instrument settings by pushing the DISPLAY SET button multiple times Recommended factory settings for IHC are SP1 of 125 C for 30 seconds SP2 of 90 C for 10 seconds SP Safety Limit of 10 C unless otherwise ...

Page 18: ...emperatures 6 Do not operate the Decloaking Chamber near a heat flame or gas source Never place the instrument directly on a stove or other heat source 7 Keep the exterior dry Remove foreign matter and water drops The heating plate sensor and the area outside of the pan must be dry and clean to work properly 8 Cool down after each use Make sure the Decloaking Chamber is cooled down after each use ...

Page 19: ...row button Pressing the Display Set button once more will cause the SP2 temperature to display You can adjust this setting higher or lower by pressing the up or down arrow button Pressing the Display Set button once more will cause the SP2 time to display You can adjust this setting higher or lower by pressing the up or down arrow button Pressing the Display Set button once more will cause the SP ...

Page 20: ...800 799 9499 60 Berry Drive Pacheco CA 94553 www biocare net 904 016 071817 ...

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