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Bio-Science CI-900 Operation Manual Download Page 31

CI-900 Instruction Manual 8/10/2015 

 

1554 NE 3

rd

 Ave, Camas, WA 98607, USA              Phone: (360) 833-8835

 

 

sales@

cid-inc

.com

                 

       

                              www.cid-inc.com

                               26 

 

Section 4: Optional Setting GC Emulation Mode Calibration Manually 

If performing a calibration injection and verifying the accuracy of the calibration, it is no longer 
necessary to carry out the following steps. If questions arise as to how the calibration is working 
and what the area being used for the peak counts is, they can be found here. However, it is 
recommended to run the standard calibration injection protocol (previous GC emulation sections 1-
3) and have the CI-900 automatically provide the peak counts.  

1. Take 3 measurements of a calibration gas standard.  
2. Determine the average of the 3 peak counts. 
3. Set the value for peak counts to the average and enter the ppb concentration in the 

calibration menu. (Setup > Calibration > press Enter to continue > C

2

H

4

 > Standard > 

Injection parameters). 

 

 
 
 
 

 

 

 

Injection parameters 

peak counts                             1103698 
concentration                         0001000 
calibrated Volume                          50.0 

Summary of Contents for CI-900

Page 1: ...CI 900 Portable Ethylene Analyzer CID Bio Science Portable Instruments for Precision Plant Measurement Inc Operation Manual...

Page 2: ......

Page 3: ...l CI 900 Complies With 89 336 EEC Electromagnetic Compatibility Directive 73 23 EEC Low Voltage Directive Compliance Standards EN 55027 RF Emissions Information Technology Equipment EN 50082 1 EMC Imm...

Page 4: ...Power 13 Measure Menu Taking a Measurement 14 Sensor Stability 14 Graph Mode 15 Monitor Mode 16 CI 900RK Using the Research Kit Fruit Chamber 19 GC Emulation Mode 21 Ethylene Sensor Zero Calibration...

Page 5: ...Electrochemical Sensor 65 Warnings 67 Technical Support 68 Customer Service 68 Frequently Asked Questions 69 Supporting Sciences References 74 CID BIOSCIENCE INC HARDWARE WARRANTY 76 APPENDIX I Mater...

Page 6: ...ng and gas chromatography analysis The intent of the CI 900 s portable design is to aid the research community in developing real world ethylene management applications for the benefit of growers proc...

Page 7: ...battery provides 4 hours of monitoring in the field Multiple configurations to accommodate in situ research and controlled atmosphere monitoring Instantaneous and accumulated measurement modes Monito...

Page 8: ...Air Sampling Rate 0 2 Liters minute Sample Rate Open Loop Monitoring 5 sec intervals Closed loop 3 min Warm up time 5 minutes C2H4 PPB Sensor Electrochemical Nominal Range 0 2 ppm Resolution 0 001 ppm...

Page 9: ...curacy 2 up to 3000 ppm Sample Cell 100 mm x 10 2 mm 3 94 x 0 4 diameter Warm up time 3 minutes Weight 172 6 g Offset Recalibration Daily Span Recalibration Bi annually calibration gas required CO2 Pe...

Page 10: ...working electrode R reference electrode C counter electrode E electrolyte Image credit doi 10 1093 aob mcs259 The CI 900 flow rate is regulated by a pump and a flow meter Additional optional sensors...

Page 11: ...analytical method is completely specific Gases present in the environment other than the target gas of a measurement may affect instrument response Interferences are not necessarily linear and may als...

Page 12: ...s been shown to filter out alcohols and produce less interference PolarCept PolarCept is the use of distilled water in conditioning Chamber In to filter out or trap some of the interfering gases Gas f...

Page 13: ...e Camas WA 98607 USA Phone 360 833 8835 sales cid inc com www cid inc com 8 PolarCept Measure Regeneration cleans the waeter PolarCept Clean Figure 5 Top diagram of measure period during use of PolarC...

Page 14: ...t Male and female quick connectors can be added to tubing and used to connect sample bags or custom chambers to the CI 900 Figure 6 Accessories and parts that arrive with the CI 900 clockwise from top...

Page 15: ...wand with flexible tubing to sample fruit or plants The fruit chamber includes a closed chamber lid and a lid with a seal to allow sampling of fruit still intact on the plant The CI 900RK includes all...

Page 16: ...remaining will be displayed on the bottom line The uptime or time the instrument has been powered on is displayed in hours minutes followed by the battery life in percent Note If the CI 900 does not p...

Page 17: ...s on off at the View Battery menu Figure 9 The battery charger setup of the CI 900 The display will flash powering off on the top line when you hit the power key Power Off will flash on the display as...

Page 18: ...ranges from 0 2000 ppm The CO2 PCT sensor is from 0 20 and does not have the same power draw To turn off on the CO2 PPM sensor 1 Go to Setup Sensor Sensor Selection 2 When asked Are you sure you want...

Page 19: ...900 will begin to warm up The CI 900 will display the Sensor Stability screen after pressing the RIGHT arrow to get into the Measure Menu The display will show the File that is open and the Flow of ga...

Page 20: ...pm As the spike or peak occurs the graph will scale to 10 000 ppm If the sample returns to 0 168 ppm the scale will remain at 10 000 ppm until the graph and the buffer are cleared The scale of the gra...

Page 21: ...e top of the display each time the data is automatically saved to the file Press the STOP key to stop the measurement and exit to the Sensor Status display Press the LEFT arrow to enter GRAPH Mode Pre...

Page 22: ...the instrument Closed Loop and enabling Chamber Out On with KMnO4 The instrument will continue to record data to the file with the mode listed as offset The instrument will allow time for ethylene to...

Page 23: ...hemical Sensor Response The lag in sensor response is a natural phenomenon for charged porous membrane electrodes The platinum electrode can be thought of as a series of cylindrical pores Each of thes...

Page 24: ...ter as well as a hydrophobic filter near the in port on the front of the CI 900 If water is found inside the tubing or filters STOP the measurement and allow the instrument to dry out Be careful no fu...

Page 25: ...concentrations the sensor fails to consume all the ethylene so KMnO4 could be used to scrub ethylene and not return it to the sample system Figure 12 Using conditioned air to replenish the fruit chamb...

Page 26: ...front of instrument for small volume samples The GC emulation mode employs a patent pending Flow Inject Analysis method to determine ethylene concentrations ranging from 0 2 20 ppmv with sample volum...

Page 27: ...not have pressure release valves please disconnect and reconnect port at end of each measurement when Results displayed to prevent pressure build up If the expected C2H4 sample s concentration is 2 pp...

Page 28: ...Enter 4 Enable or disable the Water Chamber to increase selectivity of C2H4 measurement Enable the chamber only if CO2 and O2 measurements are NOT involved Chamber In should be filled at least half fu...

Page 29: ...rt 8 Wait for processing countdown to complete from 120 seconds 9 Set the sample as your calibration standard by pressing Enter for Yes when asked set as calibration 10 Enter the concentration of the...

Page 30: ...OUT side briefly remove and replace the injection port from the CI 900 to manually release the internal pressure build up between injections Section 3 Sample Injection 12 Follow the same steps but thi...

Page 31: ...the area being used for the peak counts is they can be found here However it is recommended to run the standard calibration injection protocol previous GC emulation sections 1 3 and have the CI 900 a...

Page 32: ...e instrument and allow adequate warm up time 3 min 3 Press the Right arrow when Measure is highlighted to begin a measurement 4 Let the sensor stabilize and automatically being measuring 5 Once the me...

Page 33: ...toring or experiments longer than 24 hours The offset autocorrection duration is adjustable default 30 minutes and happens during Sensor Stability the first screen when measurement mode is initiated T...

Page 34: ...ation Mode Instructions to use Monitor Mode are found on page 16 GC Emulation Mode is available with the CI 900 Research Kit and requires the use of the inline injection port with septum To save chang...

Page 35: ...GHT arrow key to highlight the word no on the display then press the UP DOWN arrow to switch to yes Press the SAVE key to save the configuration After saving it will exit to the Setup Menu To set the...

Page 36: ...e are the PPM or the PCT which gives values in percent There is also an additional sensor available for O2 percent oxygen The optional sensors are in series meaning the gas sample will flow to the CO2...

Page 37: ...f the weight is greater than 10 0 g the instrument should be hydrated by 10 cc 1cc 1 g A positive value indicates hydration a negative value indicates dehydration of the cell A message on the display...

Page 38: ...humidity sensors inside the instrument upstream and downstream of the ethylene sensor This should only be done at the instruction of a technician RH reset serves to calibrate the relative humidity sen...

Page 39: ...The ethylene sensor calibration will drift over time Occasional recalibration of the sensors can be carried out using calibration gas in the range of each ethylene sensor For example 1 ppm C2H4 gas ca...

Page 40: ...en inject gas Record stabilized value Xf of target gas Calculate V using the formula above This should be repeated several times and the average should be taken The average calibrated volume V has bee...

Page 41: ...o ensure that the latest offset adjustment so that the unit is using the most recent zero change the Adjust enable line to Yes To return to factory calibration parameters select No Interval The offset...

Page 42: ...nu To calibrate the CO2 PPM sensor 400 ppm or 600 ppm standard CO2 gas is recommended To Calibrate the CO2 PCT 4 20 CO2 is recommended Allow the gas to run for 20 30 minutes before calibration Calibra...

Page 43: ...or is between 400 and 600 ppm The recommended concentration for the CO2 PCT sensor is 4 20 CO2 Calibrating the CO2 sensor with standardized gas is fairly simple and takes 20 30 minutes Remember to alw...

Page 44: ...t and change the value to 00050 Press Save 7 The instrument will now set the internal gain and count down from about 46 8 The final calibration values will then be displayed Press Save to save the val...

Page 45: ...o small holes in the cap of the buffer bottle 1 hole is for the intake tube and 1 hole is for the out tubing 3 Insert a short plastic tube into the cap of the buffer bottle for the out 4 Insert a long...

Page 46: ...on Connect the In tubing to the unit Place the buffer bottle in a location that has stable CO2 concentration away from operator cars furnace photosynthesizing plants Here ambient air CO2 levels should...

Page 47: ...er then O2 2 Go to Re calibration and press the right arrow You will hear the valves open and the pump begin to run 3 Use pressurized N2 to create zero O2 gas When the gas is flowing and connected to...

Page 48: ...0 is an important factor for taking good measurements The default flow rate is 200 ml min Flow rate is adjustable from 100 500 ml min A flow value below 100 ml min can negatively impact sensor functio...

Page 49: ...es a special mode where distilled water is used to filter out alcohols before the electrochemical sensor The use of distilled water in the Chamber In is referred to as PolarCept It is recommended to u...

Page 50: ...iners for distilled water and KMnO4 left and a diagram showing the flow path of the gas stream right Chamber In is on the right side of the back panel This chamber can be enabled in 2 different modes...

Page 51: ...r There is a fill line on the back of the instrument Next set the length of the measuring period in minutes This is the time the CI 900 will take to do a measurement When using the water trap a settli...

Page 52: ...the instrument when using PolarCept To avoid drawing water into the internal tubing of the CI 900 when using PolarCept 1 Fill conditioning Chamber In only to the fill line and never completely fill t...

Page 53: ...switch to the column containing the values in ppb parts per billion Use the UP DOWN arrow keys to set the desired level Press the LEFT arrow or SAVE to exit back to the Setup Menu Any changes made wil...

Page 54: ...he terminal block is designed to stay with the fan or gas control so if the CI 900 needs to be moved the terminal block can be disconnected easily Figure 17 The removable piece of the CI 900 terminal...

Page 55: ...es from standard USB connection The CI 900 Controller Software is included as part of the firmware update package http cid inc com support ci 900 support 900software named CI 900 Controller Once the p...

Page 56: ...ows the user to review the filenames and file size of any files on the CI 900 To view collected and saved data either download files via USB or eject the SD card and insert it in a computer In the Vie...

Page 57: ...s on the top line if the unit s Charging is On or Off or if the unit is connected via USB cable to a computer The QBAT value shows the battery level in hexadecimal follow immediately by the battery le...

Page 58: ...GPS system which can relay the longitude latitude and altitude of the instrument when performing measurements The View GPS screen displays the mode of the GPS sensor SEN GPGGA as well as the values f...

Page 59: ...B connection with a computer to transfer data from the CI 900 Clear a File Pressing the RIGHT arrow key when Clear is highlighted on the display enters the menu to clear files This mode allows the era...

Page 60: ...e RIGHT arrow key to delete the file Press ENTER to confirm Or to escape without deleting the file press the LEFT arrow or STOP key The display will indicate Done when the file is erased Press STOP or...

Page 61: ...a will be saved to it Pressing the SAVE key in the Create mode saves the file Should the file name be identical to another file name the instrument will display the message duplicate name on the top l...

Page 62: ...n File File01 3201 RoomA2 1022 Fruit7 0 Fruit9 2 An empty file will have a zero as the file size If the file is corrupt the file size will be a negative number Any file with a negative file size shoul...

Page 63: ...card after the measurement has been made because if any changes are made to the original file it can break the alignment of the file causing error and data loss In order to keep the file system in syn...

Page 64: ...GC emulation the CO2 and O2 concentration the temperature of the gas stream in degrees Celsius the relative humidity RH of the gas stream in percent the atmospheric pressure KPa and the flow rate of t...

Page 65: ...leaning cycle At the end of the cleaning time the ethylene level should be low below 0 2 ppm If a low ethylene concentration is not seen at the end of the cleaning period lengthen the cleaning time in...

Page 66: ...ble check the webpage for the latest compatible firmware version depending on the serial number of the CI 900 Please contact CID Bio Science technical support with questions about firmware updates Set...

Page 67: ...b win32 7 Click Install Driver to proceed installing libusb driver This could take up to 1 minute to complete Note Wait for Windows obtaining driver process to complete before clicking Install driver...

Page 68: ...lect File and Open 4 Navigate to the CI 900 ver x xx bin This is the code for the instrument 5 Follow the instructions in the software to hold down the power button 6 Release the power button when ins...

Page 69: ...en using Windows 8 please try the following 1 Hold the power button for 10 seconds and let go 2 Hold the down arrow button then press power button device s internal green LED near USB port SD card sho...

Page 70: ...ironment in which it is used the sensor cell may need to be replenished with distilled water every 2 3 weeks if Chamber In is not set to ON and filled with distilled water The electrolyte level should...

Page 71: ...o the overall sensor full weight 6 Details about the electrochemical cell status can be found in the Setup Sensor Cell Hydration menu Note The refill dead time is proportional to the R H of the measur...

Page 72: ...age rooms Damage to the analyzer can occur if the fluid reservoir level is too low or if the inlet and outlet gas connections are blocked while the pump operates This can cause false readings or irrep...

Page 73: ...your technical questions by phone or by e mail at support cid inc com CID Bio Science Inc contact information CID Bio Science 1554 NE 3rd Ave Camas WA 98607 USA Phone 800 767 0119 U S and Canada 360 8...

Page 74: ...e develop in the tubing when monitoring a fruit in the chamber a There is a protective hydrophobic filter inside the IN port on the front of the CI 900 This will prevent moisture from fully entering t...

Page 75: ...me and press save Next open the file to save measurements Go to File Open and use the arrows to highlight the newly created file If the value next to the file name is a negative number this indicates...

Page 76: ...re root nursery trees Rooms should be empty of ethylene before storing nursery trees and often growers have no way to monitor this e Monitoring ethylene emission from industrial sources Ethylene is of...

Page 77: ...it chamber to the Out port on the front of the CI 900 13 How is Chamber In used to rehydrate the electrochemical cell a The electrochemical sensor can be rehydrated by putting distilled water in Chamb...

Page 78: ...60 833 8835 sales cid inc com www cid inc com 73 17 What types of tubing materials have been found to outgas a Several types of material have been found to outgas interfering gases of the ethylene sen...

Page 79: ...and F Harren Current methods for detecting ethylene in plants Annals of Botany 2012 DOI 10 1093 aob mcs259 Golding J Shearer D McGlasson W Wyllie S Relationships between Respiration Ethylene and Aroma...

Page 80: ...A Al Mahdouri A Postharvest Responses of Malindi Cavendish Banana to Various Storage Conditions International Journal of Fruit Science volume 13 4 p 373 2013 DOI 10 1080 15538362 2013 748378 Pech J B...

Page 81: ...Bio Science Inc Material and equipment covered hereby which is not manufactured by Seller is to be covered only by the warranty of its manufacturer Seller shall not be liable to the Buyer for loss da...

Page 82: ...stance call 1 281 441 4400 SECTION 2 COMPOSTION AND INFORMATION ON INGREDIENTS Composition Name CAS by Weight Sulfuric acid 7664 93 9 9 8 Water 7732 18 5 90 2 Toxicological Data on Ingredients Sulfuri...

Page 83: ...eful to clean folds crevices creases and groin Cold water may be used If irritation persists seek medical attention Wash contaminated clothing before reusing Serious Skin Contact Wash with a disinfect...

Page 84: ...t If ingested seek medical advice immediately and show the container or the label Avoid contact with skin and eyes Keep away from incompatibles such as alkalis May corrode metallic surfaces Store in a...

Page 85: ...erature Not available Conditions of Instability Not available Incompatibility with various substances Extremely reactive or incompatible with alkalis Slightly reactive to reactive with metals Corrosiv...

Page 86: ...ON 15 OTHER REGULATORY INFORMATION Federal and State Regulations TSCA 8 b inventory Sulfuric acid Water Other Regulations OSHA Hazardous by definition of Hazard Communication Standard 29 CFR 1910 1200...

Page 87: ...ce with the CI 900 5 After sampling the headspace for 5 minutes or a set period of time of your choosing take a syringe sample and run it in the GC gas chromatograph Record result in spreadsheet progr...

Page 88: ...s of the method and how to realize if the cleaning time should be extended Procedure 1 Fill conditioning Chamber In with distilled water and clean the water using potassium permanganate in an in line...

Page 89: ...pylene value when opening closing by holding side stem while turning entire upper portion of fitting one revolution Store bags flat Do not roll or crease bags during storage Do not use bags at tempera...

Page 90: ...a handle or hanging device Although it is durable it is not intended for these uses Analyzing a Sample Bag with the CI 900 Connect the sample bag to the IN port of the CI 900 Open the sample bag to al...

Page 91: ...ve Camas WA 98607 USA Phone 360 833 8835 sales cid inc com www cid inc com 86 APPENDIX IV CI 900 Menu System Diagram Below is a map of the CI 900 ethylene analyzer menu system Press the right arrow to...

Page 92: ...00 fully assembled Optional Sensors Included Yes No C02 PPM CO2 PCT O2 PCT CALIBRATION PARAMETERS C2H4 PPB C2H4 PPM Internal offset Internal gain Calibration RH Calibration T RH slope RH offset a coef...

Page 93: ...CI 900 Instruction Manual 8 10 2015 1554 NE 3rd Ave Camas WA 98607 USA Phone 360 833 8835 sales cid inc com www cid inc com 88...

Page 94: ..._____________________ CID Serial Number s __________________________________________________ Purchase Date _____________________ Purchase Price _____________________ FOLD ON DOTTED LINE Your opinions...

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