keys.
6.3 Flow Rate Calibration
4.
To change the direction of flow, stop the pump by press-
i ng the Run/Stop key. Press the Direction key to choose
the new direction. Restart by pressing the Run/Stop key.
5.
To purge the tubing, press the Purge key. The pump will
run at 100% maximum speed. When the tubing is
primed, press the Purge key again to stop the purge
function and return to the Run mode, or press the
Run/Stop key to turn the pump off.
An uncalibrated Model EP- I Econo Pump will display pump speed as per-
cent of maximum rpm. The user may also choose to select one of three tub-
ing sizes for which calibration has been programmed at the factory. The
Model EP-1 Econo Pump also features a user calibration mode, which is
used when improved flow rate accuracy is desired, or when using non-stan-
dard size tubing. The pump must be calibrated using one of the methods
described above before a fraction collection program may be entered.
Flow Rate Calibration with Pre-Selected Tubing Sizes (0.8, 1.6,
and 3.2 mm ID)
6.2 Verification of Econo System Software Version
The Model ES-1 Econo System Controller and Model EP-1 Econo Pump
should already be set up.
The Model EP-1 Econo Pump must have software version 2.01 or
higher to control the Model EV-1 Econo Buffer Selector.
If you do not have a Model EV-1 Econo Buffer Selector, continue with
Section 6.3. Otherwise check the software version of your system:
Simultaneously press and hold the Direction Key and the "down"
Arrow key on the front panel of the Model EP-1 Econo Pump. The
four digit LED display on the front panel of the Pump should dis-
play: "v2.01" or higher.
If your pump displays a number lower than 2.01 , and you will be
using the Model EV-1 Econo Buffer Selector, your pump software
should be upgraded. Contact your local Bio-Rad representative for
details on how to upgrade software.
If your pump currently features software version 2.01 or higher ,
insure that all connections between the Model EP-1 Econo Pump,
Model ES-1 Econo System Controller, (and Model EV-1 Econo
Buffer Selector, if used) have been completed and the system power
is on.
1 2
User Calibration Procedure
1.
With the pump in Stop mode, install tubing as described
in Section 5.2.
2.
Press the key in the lower left-front of the pump panel
corresponding to the pump calibration feature, and con-
tinue pressing until the indicator light corresponding to
either 0.8, l.6, or 3.2 mm ID tubing is lit.
3.
The LED display reads ml/min for any of these pre-pro-
grammed tubing settings. If improved flow rate accuracy
is desired, see User Calibration Procedure below.
4.
The pump is now automatically calibrated for the specif-
ic tubing size. Press the Run/Stop key to start flow.
Increase or decrease the pump speed with the Arrow
keys.
1.
Install tubing as described in Section 5.2. Connect pump
inlet to a container of water or buffer. Connect a length
of tubing to the pump outlet.
2.
Run pump to purge air from tubing, then stop pump.
Place the end of the outlet tubing in an empty graduated
cylinder.
3.
Press Calibration key until "CAL" is displayed. Press the
Run/Stop key. Pump will run for 5 minutes at 25% of
maximum speed, and the display will count down time.
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