4.
When pump stops, read the volume of fluid on the grad-
uated cylinder. Using the Arrow keys, enter this amount
on the display. Alternatively, when a suitable volume of
liquid has been collected, pressing Run/Stop will turn
off the timer and stop the pump. Enter volume collected
as described.
5.
When the value on the display matches the volume col-
lected in the graduated cylinder, press Calibration. The
"CAL" indicator light will glow steadily, indicating that
the pump is calibrated. DO NOT select a tubing size at
this point, even if you are using one of the standard
sizes. The pump will "remember" the calibration key
even if it is switched off or disconnected from the power
line.
6.
Set desired flow rate using arrow keys.
Note: Flow rate depends on condition of tubing, platen
adjustment, and back pressure. For maximum flow rate
accuracy it may be necessary to recalibrate as the tubing
ages, when the tubing is replaced, if the platen is read-
justed, or if back pressure changes significantly.
6.4 Simple Time-Based Collection with the Model
2110 Fraction Collector
In addition to its features as a stand-alone pump, the Model EP-1 Econo
Pump is capable of controlling the Model 2110 Fraction Collector as well as
other fraction collectors (for operation with collectors other than the Model
2110 Fraction Collector, see Appendix B). The pump must be calibrated in
order to run a fraction collection scheme. See Section 5.3 for calibration.
Use Econo System Cable #l to connect the I/O socket on the rear panel of
the fraction collector to the 8-pin connector on the rear of the Model EP-1
Econo Pump (see Figure 4.2).
1.
Press the Fraction Collector key on the pump front panel
to enter the fraction collector Edit mode. Note that the
fraction size and ml indicator lights are flashing,
prompting entry of fraction size. Enter the fraction size
in 0.l ml increments using the Arrow keys. After frac-
tion size is selected, press the Fraction Collector key
once more to confirm the fraction size.
14
2.
3.
*
Note: The Econo Pump will automatically shut off upon
reaching the total run volume (V t ).
4.
5.
6.
Enter a void volume (V) in I ml increments using the
Arrow keys. This feature allows the user to begin col-
lecting fractions only after a pre-set volume of liquid
(i.e., the void volume) has passed through the column.
Press the Fraction Collector key to confirm this value. If
a void volume is not desired, enter 0.
Note: The optional Model SV-3 Diverter Valve (catalog
number 731-8235) is required to use the void volume
feature of the Model EP-1 Econo Pump.
Select the total run volume (V) in 1 ml increments using
the Arrow keys.* If zero is entered and confirmed, the
fraction collection scheme is disabled. To resume data
entry, see Step 1 above. All values previously entered
remain in the memory until other values are entered to
replace them.
After selecting a
V
value, press the flashing Fraction
Collection key to enter the value. The flashing LED dis-
play shows the estimated number of fractions to be col-
lected.
Press the flashing Fraction Collection key once more to
enable the fraction collector. To actuate the fraction col-
lector, press the Program Run key after starting the
pump. (The fraction collector can be programmed with
the pump running or stopped.) After the fraction collec-
tion program is initiated, the LED display shows the
progression of the fraction collection scheme in minutes,
to the nearest tenth of a minute.
While a program is running, the user can use the Arrow
keys to display the ml of output, the progression of the
collection in min, the flow rate in ml/min, or the percent
of total run-time. If the Econo Pump is connected to the
Econo System Controller and the Gradient Former is
enabled, % buffer B can also be viewed while a program
is running.
15
