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Section 5
Instrument Setup and Use for
96-well Plates
Tubing provided is 4 ft. long and must be cut in appropriate pieces before
proceeding.
Vacuum setup conditions
1.
Cut tubing into 3 pieces of appropriate length.
2.
Use one piece of tubing to connect the vacuum source to the
right side
of the vacuum regulator.
3.
Use another piece of tubing to connect the left side of the vacuum
regulator to the sidearm of the filter flask.
4.
Place a rubber stopper
with hole
into the mouth of the filter flask. Insert
a serological pipette (or comparable) into the hole of the stopper.
5.
Finally, use the last piece of tubing to connect the filter flask to the nozzle
of the manifold.
Note:
The Aurum vacuum regulator is strongly recommended to enable
full control of the negative pressure within the manifold.
Vacuum purification conditions
6.
Remove the manifold top and place the A stage in the depression in the
center of the manifold base. The etched letter A should be facing up.
7.
Place the waste collection tray on top of the A stage. Replace the
manifold top on the base, ensuring complete and uniform contact
between the manifold top and base.
8.
Place the desired 96-well plate on the manifold top, and follow the
instructions in the protocol for the appropriate purification kit.
Vacuum elution conditions
9.
When eluting from the purification plate, remove the A stage and waste
collection tray from the Aurum vacuum manifold. Place the B stage on the
manifold base, with the etched letter B facing up. Place a clean 96-well
microtiter collection plate securely on top of the B stage and replace the
manifold top.
10. After finishing the elution, rinse the Aurum vacuum manifold with water,
and air dry or wipe with paper towels.
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Fig. 2. Instrument setup and use for plate and column
processing.
2a: 96-well purification, 2b: 96-well elution, 2c: CAP
purification
Fig. 3. Vacuum Setup Conditions
a.
b.
c.
