BIO RAD Aurum, Instruction Manual

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Section 10
Ordering Information
Catalog # Description
732-6470
A Au urru um m v va ac cu uu um m m ma an niiffo olld d, includes column adaptor plate,
4 replacement luer caps, A and B stages, waste collection
tray, vacuum regulator and gauge, tubing, protocol overview
and instruction manual.
732-6400
A Au urru um m p plla as sm miid d m miin nii k kiitt, 100 preps, includes 100 plasmid
binding columns, 100 capless collection tubes (2.0 ml),
100 capped sample tubes (1.5 ml), reagents, protocol
overview and instruction manual.
732-6440 A Au urru um m p plla as sm miid d m miid dii k kiitt, 20 preps, includes 20 lysate
filtration columns, 20 plasmid binding columns, 20 adaptor
caps, reagents, protocol overview and instruction manual.
732-6450 A Au urru um m p plla as sm miid d m ma ax xii k kiitt, 10 preps, includes 10 lysate
filtration columns, 10 plasmid binding columns, 10 adaptor
caps, reagents, protocol overview and instruction manual.
732-6460 A Au urru um m p plla as sm miid d 9 96 6 k kiitt, 2 x 96 well preps, includes 2 grow
blocks, 2 grow membranes, 2 binary units (2 lysate filtration
plates and 2 plasmid binding plates), 2 collection plates,
reagents, protocol overview and instruction manual.
Problem Possible Cause Possible Solution
Worn manifold gasket Replace gasket
Incorrect negative Vacuum source connected Connect vacuum
pressure from vacuum to left side of vacuum to right side of vacuum
regulator
Clogging of purification Insufficient negative See Problem “Insuffi-
plate or CAP columns pressure in vacuum cient negative pressure
manifold in vacuum manifold”
above
Purification problem Refer to troubleshooting
seated properly section of appropriate
kit
Plasmid prep performs Proteinaceous debris in See Problem Low
poorly in enzymatic prep (if A
260/280
< 1.7) A
260/280
ratio
reactions
Plasmid is degraded Use endA- strain (e.g.
JM109, DH5
α
, XL1-
Blue)
Ethanol contamination in Add 1-3 minutes to the
prep (eluate volumes purge time after the
> 55 ml) wash step
Remove all traces of
Wash
Solution from the drip
directors of the plasmid
binding plate by blotting
on paper towels prior to
elution