2.5 Priming the Gradient Pumps and Equilibrating
the UNO Q1 Column
Be sure the gradient pumps are stopped and the inject valve is in
the purge position
. Re-prime and purge pumps A and B as described in
Section 2, step 1 of this manual.
Set the inject valve to position L (Load). Set the flow rate to
2.0 ml/min. Set the UV range to 0.1 AUFS and the conductivity range to
100 mS/cm.
1. Wash the column with 6.5 ml (5 column volumes) of buffer B at
2 ml/min to remove the column storage solution.
2. Equilibrate the column with13 ml (10 column volumes) of 100%
buffer A.
The conductivity monitor on the status bar should now read
approximately 3 mS/cm.
2.6 Creating a Method
From the Manual Control screen, click the
New Method
toolbar
button. You will see a dialog box asking for a method name (input Starter
Kit Separation–Auto). Use the keyboard tab key to go to the Method
Description and Method Author fields. Make an entry in these fields if
desired and click the
OK
button. You will now see the Setup Editor.
Programming the Setup Editor
This information entered in the Setup Editor is important for the
subsequent separation method. The buttons grouped in the left hand box
(Available Devices) shows all the devices that could theoretically be
connected to the BioLogic Duo-Flow System.
The list of items in the right hand box (Devices in Setup) identifies
those devices you wish to use in the subsequent method. The initial default
Devices in Setup are a UV detector and a conductivity monitor as these
come as standard with the BioLogic Duo-Flow System. The default devices
in Setup can be changed by choosing
Save setup as default
from the
Data pull-down menu.
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